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Molecular characterization of porcine rotavirus C in pigs with gastroenteritis in Thailand, 2011 – 2016

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104 days ago
Molecular characterization of porcine rotavirus C in pigs with gastroenteritis in Thailand, 2011 – 2016 https://t.co/FMIoFdVoEf
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Supplemental Information

Multiple sequence alignments of the eight variable regions (VR1–VR8) in each RVC genotypes

Different colors correspond to hypervariable sites. Dashes indicate gap;. · indicates identical amino acids. The N-glycosylation site between residues 67 and 69 is presented in red. The color shading indicates the mutations among current strains and RVC prototype or reference strains (bold), whereas pink, purple, yellow, light blue, green, orange and turquoise colors stand for those residues located within VR-2 to VR-8, respectively. The whole VR-1 region and the first seven residues of the VR-2 region (between residues 31 and 37) were non-applicable.

DOI: 10.7287/peerj.preprints.3515v1/supp-1

The raw data of RVC detection

Total case submission, RVC positive case and RVC genotype (VP7)

DOI: 10.7287/peerj.preprints.3515v1/supp-2

Sequences for RVC VP7

Sequences for RVC VP7

DOI: 10.7287/peerj.preprints.3515v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Supansa Tuanthap conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Cherdpong Phupolphan performed the experiments.

Supol Luengyosluechakul conceived and designed the experiments, performed the experiments.

Usanee Duang-in performed the experiments.

Apiradee Theamboonlers analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Sompong Vongpunsawad analyzed the data, reviewed drafts of the paper.

Suphot Wattanaphansak conceived and designed the experiments.

Alongkorn Amonsin reviewed drafts of the paper.

Yong Poovorawan conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Animal Care and Use Committee (IACUC) (animal use protocol number 1731020) of Chulalongkorn University, Bangkok, Thailand.

Funding

This work was supported by the 100th Anniversary Chulalongkorn University Fund for doctoral scholarship to Supansa Tuanthap in Inter-Department Program of Biomedical Sciences, Faculty of Graduate School, Chulalongkorn University, Bangkok, Thailand, the National Research Council of Thailand, the Research Chair Grant from NSTDA (P-15-50004) and the Center of Excellence in Clinical Virology (GCE 59-009-30-005). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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