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Proanthocyanidins in seed coat’s tegmen and endospermic cap inhibit seed germination in the bioenergy plant Sapium sebiferum

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RT @Xenpaper: Proanthocyanidins in seed coat’s tegmen and endospermic cap inhibit seed germination in the bioenergy plant Sapium sebiferum…
100 days ago
Proanthocyanidins in seed coat’s tegmen and endospermic cap inhibit seed germination in the bioenergy plant Sapium sebiferum https://t.co/1VqlUpFB9U
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Supplemental Information

Impact of sulfuric acid stratification on seed germination of Sapium sebiferum

A, B, C, D, E, F and G are 0-(control), 10-, 20-, 30-, 40-, 50- and 60 minutes incubation in concentrated sulfuric acid respectively. Ten seeds of every treatment were sown in each 10×10 cm pot with 5 replicates. Photographs were taken after one month of seed sowing.

DOI: 10.7287/peerj.preprints.3514v1/supp-1

Effect of SCE and PAs on seed germination of S. Sebiferum

A, 0.5×MS (control). B, 0.3% SCE+0.5×MS. C, 0.1% PAs+0.5×MS. For each treatment, twelve seeds were sown in a 9 diameter cm Petri plate separately. All treatments were replicated 5 times. The Photographs were taken on the 7th day of imbibition. Bar = 2cm

DOI: 10.7287/peerj.preprints.3514v1/supp-2

Germination promoters recovered the seed germination sown in half MS supplemented with seed coat extract

A, control (sterile water). B, 50 μΜ GA3. C, 50 μΜ NDGA. D, 20 mM H2O2 and E, 0.4% KNO3 priming overnight at room temperature and the primed seed of all treatments were grown separately on 0.3% SCE+0.5×MS in 9 cm Petri plates (12 seeds per plate) for 7 days. These photographs were taken on the 7th day of imbibition. Bars = 1 cm >

DOI: 10.7287/peerj.preprints.3514v1/supp-3

List of primers used for qPCR

DOI: 10.7287/peerj.preprints.3514v1/supp-4

Full length genes sequences with translated CDS

DOI: 10.7287/peerj.preprints.3514v1/supp-5

water uptake percentage

Time, Scarification time. WUT, water uptake(%). Data was take 72 hours after imibibition

DOI: 10.7287/peerj.preprints.3514v1/supp-6

Effect of H2SO4 scarification time on seed germination

Treatment, H2SO4 scarification time. Germination, germination(%)

DOI: 10.7287/peerj.preprints.3514v1/supp-7

Root and shoot length of seedlings produced from H2SO4 scarified seed

Time, H2SO4 scarification time. Root, root length (cm). Shoot, shoot length (cm)

DOI: 10.7287/peerj.preprints.3514v1/supp-8

Effect of H2SO4 scarification time on PAs contents of seed coat

IT, H2SO4 scarification time. PAS, PAs contents (%) of seed coat after H2SO4 scarification

DOI: 10.7287/peerj.preprints.3514v1/supp-9

Datasheet of fig.5, 6 and 7

Data shows the delta C value of each gene. IDS, imbibition days

DOI: 10.7287/peerj.preprints.3514v1/supp-10

PAs contents in seed coat extract, testa and tegemen

DOI: 10.7287/peerj.preprints.3514v1/supp-11

PAs and SCE effect on seed germination

SCE and PAs were added in half MS. Data was taken after 7 days of imbibition

DOI: 10.7287/peerj.preprints.3514v1/supp-12

Data sheet of Figure 8 Impact of germination promoters

Germination, germination (%)

DOI: 10.7287/peerj.preprints.3514v1/supp-13

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Faheem Afzal Shah conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, seed collection, Taking photographs.

Jun Ni conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, taking photographs.

Jing Chen performed the experiments.

Qiaojian Wang performed the experiments, contributed reagents/materials/analysis tools, seed collection.

Wenbo Liu performed the experiments.

Xue Chen analyzed the data, contributed reagents/materials/analysis tools.

Caiguo Tang analyzed the data, contributed reagents/materials/analysis tools.

Songling Fu conceived and designed the experiments, wrote the paper, reviewed drafts of the paper.

Lifang Wu conceived and designed the experiments, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Genes sequences are given in Supplemental data1

Funding

This work was funded by Anhui Natural Science Foundation (1708085QC70), the National Natural Science Foundation of China (11375232 & 31500531), the Science and Technology Service program of Chinese Academy of Sciences (KFJ-STS-ZDTP-002&KFJ-SW-STS-143-4), the Grant of the President Foundation of Hefei Institutes of Physical Science of Chinese Academy of Sciences (YZJJ201502 & YZJJ201619), the major special project of Anhui Province (16030701103), research and technology project of Anhui province (1501031079). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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