Proteomic identification of Galectin-11 and 14 ligands from Haemonchus contortus

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1 Department of Animal, Plant and Soil Science and Centre for AgriBioscience (AgriBio), La Trobe University, Bundoora, Victoria, Australia
2 Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia
3 School of Applied and Biomedical Sciences, Federation University, Churchill, Australia
4 Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, Australia
5 Department of Biochemistry & Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Australia
DOI
10.7287/peerj.preprints.3473v1
Published
Accepted
Subject Areas
Biochemistry, Parasitology, Veterinary Medicine
Keywords
Mass spectrometry, Host-parasite interactome, galectin-14., Haemonchus contortus, galectin-11, Gastrointestinal parasite, galectin
Copyright
© 2017 Sakthivel et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Sakthivel D, Swan J, Preston S, Shakif-Azam M, Faou P, Jiao Y, Downs R, Rajapaksha H, Gasser R, Piedrafita D, Beddoe T. 2017. Proteomic identification of Galectin-11 and 14 ligands from Haemonchus contortus. PeerJ Preprints 5:e3473v1

Abstract

Haemonchus contortus is the most pathogenic nematode of small ruminants. Infection in sheep and goats results in anaemia that decreases animal productivity and can ultimately cause death. The involvement of ruminant-specific galectin-11 (LGALS-11) and galectin-14 (LGALS-14) has been postulated to play important roles in protective immune responses against parasitic infection; however, their ligands are unknown. In the current study, LGALS-11 and LGALS-14 ligands in H. contortus were identified from larval (L4) and adult parasitic stages extracts using immobilised LGALS-11 and LGALS-14 affinity column chromatography and mass spectrometry. Both LGALS-11 and LGALS-14 bound more putative protein targets in the adult stage of H. contortus (43 proteins) when compared to the larval stage (2 proteins). Of the 43 proteins identified in the adult stage, 34 and 35 proteins were bound by LGALS-11 and LGALS-14, respectively, with 26 proteins binding to both galectins. Interestingly, hematophagous stage-specific sperm-coating protein and zinc metalloprotease (M13), which are known vaccine candidates, were identified as putative ligands of both LGALS-11 and LGALS-14. The identification of glycoproteins of H. contortus by LGALS-11 and LGALS-14 provide new insights into host-parasite interactions and the potential for developing new interventions.

Author Comment

This is a submission to PeerJ for review.