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Supplemental Information

Figure S1: Newly developed fusion primer sets (BF2+BR2), suitable for tagging 288 individual wells

DOI: 10.7287/peerj.preprints.3456v4/supp-1

Figure S2: Base composition of the inline tagging region

DOI: 10.7287/peerj.preprints.3456v4/supp-2

Figure S3: Hamming distance between tags for all fusion primers

DOI: 10.7287/peerj.preprints.3456v4/supp-3

Figure S4: Levenshtein distance between tags for all fusion primers

DOI: 10.7287/peerj.preprints.3456v4/supp-4

Script S1: R script used for randomly generating inline barcodes for the given primer sets (includes visualization, as shown in Figure S3 and Figure 3)

DOI: 10.7287/peerj.preprints.3456v4/supp-5

Table S1: Table providing an overview of proposed tagging combinations (as shown in Figure 3)

DOI: 10.7287/peerj.preprints.3456v4/supp-6

Manuscript file for providing feedback

(Pleas use track changes)

DOI: 10.7287/peerj.preprints.3456v4/supp-7

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Vasco Elbrecht conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Dirk Steinke authored or reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

primer sequences and scripts to generated them are provided as supporting information


V.E. and D.S. are supported by funding through the Canada First Research Excellence Fund. This work represents a contribution to the ‘Food From Thought’ research program and the EU Action ‘DNAqua-Net’ (CA15219). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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