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Supplemental Information

Table S1. Primers used for multi-transgenic identification

DOI: 10.7287/peerj.preprints.3437v1/supp-1

Supplemental Raw Figures for Figure 1 PCR

DOI: 10.7287/peerj.preprints.3437v1/supp-2

Comparison of plasma glucose levels in single-transgenic, dual-transgenic and triple-transgenic mice

DOI: 10.7287/peerj.preprints.3437v1/supp-3

Body weights and IPGTT data indicating insulin resistance of triple-transgenic mice

DOI: 10.7287/peerj.preprints.3437v1/supp-4

Anatomical analysis of triple-transgenic mice

DOI: 10.7287/peerj.preprints.3437v1/supp-5

Liver tissue (hepatology) pathology

DOI: 10.7287/peerj.preprints.3437v1/supp-6

Pathology of pancreatic islets of Langerhans

DOI: 10.7287/peerj.preprints.3437v1/supp-7

Serological parameters of mouse models related to pre-diabetes

DOI: 10.7287/peerj.preprints.3437v1/supp-8

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Siyuan Kong performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Jinxue Ruan performed the experiments, analyzed the data.

Kaiyi Zhang reviewed drafts of the paper.

Bingjun Hu performed the experiments, analyzed the data.

Yuzhu Cheng performed the experiments, analyzed the data.

Yubo Zhang conceived and designed the experiments.

Shulin Yang conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Kui Li conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All procedures were approved by the Animal Care and Use Committee of the Germplasm Resource Center (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China) (permit no. ACGRCM 2013-035).


The present study was supported by the National Natural Science Foundation of China (grant no. 31372276), the State Key Laboratory of Animal Nutrition (grant no. 2004DA125184G1602), the National Basic Research Program of China (grant no. 2015CB943100), the Agricultural Science and Technology Innovation Program (grant nos. ASTIP-IAS05 and ASTIP-IAS-TS-4), and the Shenzhen Special Fund for Strategic Emerging Industries Development (grant no. CXZZ20140504105105077). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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