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De novo assembly and identification of antennal transcriptome reveals an olfactory system in Heortia vitessoides (Lepidoptera: Crambidae)

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PJpreprint: De novo assembly and identification of antennal transcriptome reveals an olfactory system in Heortia vitessoides (Lepidoptera: Crambidae) https://t.co/4O82FNcDO8
16 days ago
De novo assembly and identification of antennal transcriptome reveals an olfactory system in Heortia vitessoides (Lepidoptera: Crambidae) https://t.co/Z2FsakjBQv
De novo assembly and identification of antennal transcriptome reveals an olfactory system in Heortia vitessoides (Lepidoptera: Crambidae) https://t.co/3lpy4KPdXp Olfactory systems in insects are used to detect volatile chemical odors, and play crucial roles in survival…
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Supplemental Information

Gene ontology classifications of unigenes

The results are assigned into three main categories: biological process, cellular component, and molecular function.

DOI: 10.7287/peerj.preprints.3433v1/supp-1

Number of H. vitessoides unigenes in the 25 clusters of eukaryotic orthologous group (KOG) functional classes

DOI: 10.7287/peerj.preprints.3433v1/supp-2

Distribution of H. vitessoides transcripts among the Kyoto Encyclopedia of Genes and Genomes (KEGG)

DOI: 10.7287/peerj.preprints.3433v1/supp-3

Phylogenetic tree of gustatory receptors (GRs) from lepidopteran insects

Hvit, H. vitessoides; Cpun, C. punctiferalis; Hass, H. assulta; Harm, H. armigera. GenBank accession numbers and amino acid sequences used for the tree are given in Table S1.

DOI: 10.7287/peerj.preprints.3433v1/supp-4

Phylogenetic tree of sensory neuron membrane proteins (SNMPs) from lepidopteran insects

Hvit, H. vitessoides; Ofur, O. furnacalis; Csup, C. suppressalis; Cmed, C. medinalis. GenBank accession numbers and amino acid sequences used for the tree are given in Table S1.

DOI: 10.7287/peerj.preprints.3433v1/supp-5

Expression abundance of three gustatory receptors (GRs) in the H. vitessoides antennal transcriptome dataset

The gene expression abundance is indicated as the fragments per kilobase per million mapped fragments (FPKM) values.

DOI: 10.7287/peerj.preprints.3433v1/supp-6

Expression abundance of two sensory neuron membrane proteins (SNMPs) in the H. vitessoides antennal transcriptome dataset

The gene expression abundance is indicated as the fragments per kilobase per million mapped fragments (FPKM) values.

DOI: 10.7287/peerj.preprints.3433v1/supp-7

GenBank accession numbers and amino acid sequences of insect OBPs, CSPs, ORs, IRs and SNMPs used for the phylogenetic analyses

DOI: 10.7287/peerj.preprints.3433v1/supp-8

Candidate GRs and SNMPs identified in H. vitessoides antennae

DOI: 10.7287/peerj.preprints.3433v1/supp-9

Nucleotide sequences and amino acid sequences of HvitOBPs, HvitCSPs, HvitORs, HvitIRs, HvitGRs and HvitSNMPs obtained in the study

DOI: 10.7287/peerj.preprints.3433v1/supp-10

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Jie Cheng conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Chun-Yan Wang prepared figures and/or tables.

Zi-Hao Lyu performed the experiments.

Jing-Xiang Chen performed the experiments.

Tong Lin contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

The raw data used for transcriptomic assemblies was downloaded from NCBI SRA with the following accession numbers: Female antennae: SRR5980134; Male antennae: SRR5980139.

Funding

This work was supported by the National Natural Science Foundation of China (No. 31470653) and the Natural Science Foundation of Guangdong Province (No. 2015A030313416). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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