De novo assembly and identification of antennal transcriptome reveals an olfactory system in Heortia vitessoides (Lepidoptera: Crambidae)
- Published
- Accepted
- Subject Areas
- Entomology, Genomics
- Keywords
- Heortia vitessoides, Antennae, Transcriptome analysis, Olfactory genes
- Copyright
- © 2017 Cheng et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- (2017) De novo assembly and identification of antennal transcriptome reveals an olfactory system in Heortia vitessoides (Lepidoptera: Crambidae) PeerJ Preprints 5:e3433v1 https://doi.org/10.7287/peerj.preprints.3433v1
Abstract
Olfactory systems in insects are used to detect volatile chemical odors, and play crucial roles in survival, reproduction, and mediating key behaviors. Here, RNA sequencing technology was used to sequence and assemble the antennal transcriptome of Heortia vitessoides, a defoliating pest in Aquilaria sinensis (Loureiro) Sprenger forests and a non-model species with no genomic resources. Analysis of the transcriptome of female and male antennae generated 22.16 gigabases of genomic data, from which 52,383 unigenes were assembled. We identified 80 candidate olfactory genes: eight for odorant binding proteins (OBPs), 14 for chemosensory proteins (CSPs), 35 odorant receptors (ORs), 18 ionotropic receptors (IRs), three gustatory receptors (GRs), and two for sensory neuron membrane proteins (SNMPs). Furthermore, phylogenetic trees and fragments per kilobase of transcript per million fragments mapped (FPKM) were used to analyze these olfactory genes. This study is the first comprehensive antennal transcriptome analysis for H. vitessoides, and these novel olfactory genes will increase understanding of the molecular mechanism of chemoreception and further contribute to exploring strategies to manage this insect.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
Gene ontology classifications of unigenes
The results are assigned into three main categories: biological process, cellular component, and molecular function.
Number of H. vitessoides unigenes in the 25 clusters of eukaryotic orthologous group (KOG) functional classes
Distribution of H. vitessoides transcripts among the Kyoto Encyclopedia of Genes and Genomes (KEGG)
Phylogenetic tree of gustatory receptors (GRs) from lepidopteran insects
Hvit, H. vitessoides; Cpun, C. punctiferalis; Hass, H. assulta; Harm, H. armigera. GenBank accession numbers and amino acid sequences used for the tree are given in Table S1.
Phylogenetic tree of sensory neuron membrane proteins (SNMPs) from lepidopteran insects
Hvit, H. vitessoides; Ofur, O. furnacalis; Csup, C. suppressalis; Cmed, C. medinalis. GenBank accession numbers and amino acid sequences used for the tree are given in Table S1.
Expression abundance of three gustatory receptors (GRs) in the H. vitessoides antennal transcriptome dataset
The gene expression abundance is indicated as the fragments per kilobase per million mapped fragments (FPKM) values.
Expression abundance of two sensory neuron membrane proteins (SNMPs) in the H. vitessoides antennal transcriptome dataset
The gene expression abundance is indicated as the fragments per kilobase per million mapped fragments (FPKM) values.
