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Global epigenetic and metabolic changes accompany the alterations in fruit size and shape of Cucurbita pepo L. intra-species grafting

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20 days ago
Global epigenetic and metabolic changes accompany the alterations in fruit size and shape of Cucurbita pepo L.… https://t.co/YFIWJbuiD2
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Supplemental Information

Example of electropherograms produced in the ABI 3730xl capillary electrophoresis automated sequencer

Supplementary Figure 1: Example of electropherograms produced in the ABI 3730xl capillary electrophoresis automated sequencer. Individual electropherograms correspond to the two control plants (‘Munchkin’ top left panel and ‘Big Moose’ top right panel) and to two heterografted plants [‘Big Moose’ (RS) x ‘Munchkin’ (SC); bottom left panel and one ‘Munchkin’ (RS) x ‘Big Moose’ (SC); bottom right panel]. Each grafted plant is represented by two electropherograms (panel) corresponding to the two isoschizomer combinations employed for MSAP analysis (EcoRI/HpaII; HpaII and EcoRI/MspI; MspI). In each electropherogram the horizontal axis represents fragment size (bp) while the vertical axis represents relative peak (fragment) fluorescence intensity. The blue horizontal line indicates range of fragment size (150–500bp; not shown).

DOI: 10.7287/peerj.preprints.3389v1/supp-1

Sequences of adapters used for the EcoRI and HpaII/MspI ligation step, and for the pre-selective (EcoRI+1, HpaII/MspI+1) and selective (EcoRI+3, HpaII/MspI+3) amplifications

Table S1. Sequences of adapters used for the EcoRI and HpaII/MspI ligation step, and for the pre-selective (EcoRI+1, HpaII/MspI+1) and selective (EcoRI+3, HpaII/MspI+3) amplifications.

DOI: 10.7287/peerj.preprints.3389v1/supp-2

MSAP raw data

MSAP raw data

DOI: 10.7287/peerj.preprints.3389v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Aliki Xanthopoulou conceived and designed the experiments, performed the experiments, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Aphrodite Tsaballa wrote the paper, reviewed drafts of the paper.

Ioannis Ganopoulos conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Aliki Kapazoglou analyzed the data, wrote the paper, reviewed drafts of the paper.

Evangellia Avramidou performed the experiments, prepared figures and/or tables.

Filippos Aravanopoulos analyzed the data, wrote the paper, reviewed drafts of the paper.

Theodoros Moysiadis analyzed the data, wrote the paper, prepared figures and/or tables.

Maslin Osathanunkul analyzed the data, wrote the paper.

Athanasios Tsaftaris contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Andreas Doulis wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Apostolos Kalivas analyzed the data.

Eirini Sarrou performed the experiments, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Stefan Martens analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Irini Nianiou- Obeidat conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Panagiotis Madesis conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

The raw data is too large to be made available online.

Funding

The authors received no funding for this work.


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