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Protective effect of Rhus coriaria fruit extracts against hydrogen peroxide-induced oxidative stress in muscle progenitors and zebrafish embryos

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23 days ago
Protective effect of Rhus coriaria fruit extracts against hydrogen peroxide-induced oxidative stress in muscle… https://t.co/h2ZV7CVFNM
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Supplemental Information

Cell cycle distribution of LHCN-M2 after treatment with sumac crude extract and ethyl acetate fraction

Histogram showing the percentages of cells at various phases of cell cycle. Values are means of three independent experiments.

DOI: 10.7287/peerj.preprints.3383v1/supp-2

Hatching of zebrafish embryos treated with sumac crude extract and ethyl acetate fraction at 96hpf

Hatching rates of the embryos were completed after 96 hpf of crude extract (A) and ethyl acetate fraction (B) exposure. This experiment was performed 3 times. The significance (*p ≤ 0.05, **p≤ 0.01& *** p<0.001) of embryos hatching in exposed embryos with respect to untreated embryos (CTRL).

DOI: 10.7287/peerj.preprints.3383v1/supp-3

Viability of zebrafish embryos after exposure to H2O2

Effect of H2O2 on zebrafish embryos viability after treatment with the following concentrations 10-3, 5. 10-3, 7.5 10-3, 10-2, 2. 10-2, 2.5 10-2, 2.5 10-2, 3. 10-2, 5.10-2, 7.5 10-2, 10-1. Mean values with S.D. are indicated. This experiment was performed in duplicates and repeated 3 times.

DOI: 10.7287/peerj.preprints.3383v1/supp-4

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Fadia Najjar conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Francine Rizk conceived and designed the experiments, analyzed the data, reviewed drafts of the paper.

Gilles Carnac conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Rim Nassar performed the experiments, reviewed drafts of the paper.

Sara Jabak performed the experiments, prepared figures and/or tables, reviewed drafts of the paper.

Anatoly Petrovich Sobolev performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Yara Bou Saada performed the experiments, prepared figures and/or tables, reviewed drafts of the paper.

Marwan El Sabban conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Aline Hamade conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The ethical panel of the department of Life & Earth Sciences of the Lebanese University - Faculty of Sciences/Fanar fully approved this research.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

As we already mentioned in the Material and Methods section, the fresh fruit from Rhus coriaria L. plant was collected from South Lebanon. No field permit was required because the fresh fruits were collected from a private field (that the corresponding author owns). Fresh fruit was only collected from the field and all experimental procedures were done in the laboratory.

Data Deposition

The following information was supplied regarding data availability:

The raw data has been provided as a Supplemental File.

Funding

The study was funded by the National Council for Scientific Research (CNRS)-Lebanon, the Lebanese University- Lebanon, the Doctoral School of Sciences and Technology (EDST)-Lebanon, Lebanese University, and Edde Association-Lebanon. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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