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Fine grained compositional analysis of Port Everglades Inlet microbiome using high throughput DNA sequencing

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Fine grained compositional analysis of Port Everglades Inlet microbiome using high throughput DNA sequencing https://t.co/uNWVr4Dq3z
Fine grained compositional analysis of Port Everglades Inlet microbiome using high throughput DNA sequencing https://t.co/OA9EjRYWvm
30 days ago
Fine grained compositional analysis of Port Everglades Inlet microbiome using high throughput DNA sequencing https://t.co/Co13Cj5sqq
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Supplemental Information

Table of Adonis test results

The Adonis (PERMANOVA) results calculated using Bray-Curtis dissimilarity index

DOI: 10.7287/peerj.preprints.3369v1/supp-1

Percent abundance values and rainfall and temperature metadata values over time

Shows an interesting inverse trend in percent abundance levels of Cyanobacteria and Proteobacteria across a 1-year timespan in relation to one another and to rainfall and temperature data.

DOI: 10.7287/peerj.preprints.3369v1/supp-2

Classification of the top 50 OTUs from rank abundance curve

This spreadsheet contains the remaining 40 classifications of the OTUs depicted in Figure 1

DOI: 10.7287/peerj.preprints.3369v1/supp-3

Mapping file containing metadata for samples

The mapping file used to link the metadata with sample. This file is necessary for downstream bioinformatic analysis of sequence data

DOI: 10.7287/peerj.preprints.3369v1/supp-4

Linear regression analysis raw data

Excel spreadsheet containing the raw data used for the linear regression analysis in SAS.

DOI: 10.7287/peerj.preprints.3369v1/supp-5

Raw alpha diversity metrics

Contains the raw data of the alpha diversity metrics obtained using the Phyloseq package in R

DOI: 10.7287/peerj.preprints.3369v1/supp-6

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Lauren O'Connell conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Song Gao contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Donald McCorquodale contributed reagents/materials/analysis tools, wrote the paper.

Jay Fleisher analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables.

Jose V Lopez conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The Port Everglades Inlet Microbiome sequences have been deposited in the NCBI database under BioProject accession number: PRJNA413618 and under the Sequence Read Archive (SRA) accession IDs of SRR6147998- SRR6148078.

Data Deposition

The following information was supplied regarding data availability:

1.) National Center for Biotechnology Information database in the Short Read Archive (SRA) database

2.) BioProject ID: PRJNA413618/ SRA accession IDs: SRR6147998- SRR6148078

3.) https://trace.ncbi.nlm.nih.gov/Traces/study/?acc=SRP119576

Funding

The work was supported by a President's Grant through Nova Southeastern University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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