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A historical legacy of antibiotic utilization on bacterial seed banks in sediments

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30 days ago
RT @MicrobiomDigest: Preprint: A historical legacy of antibiotic utilization on bacterial seed banks in sediments https://t.co/UIBAtGuQmX
RT @MicrobiomDigest: Preprint: A historical legacy of antibiotic utilization on bacterial seed banks in sediments https://t.co/UIBAtGuQmX
Preprint: A historical legacy of antibiotic utilization on bacterial seed banks in sediments https://t.co/UIBAtGuQmX
33 days ago
A historical legacy of antibiotic utilization on bacterial seed banks in sediments https://t.co/Np8W90OZPl
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Supplemental Information

Total abundance of tetracycline and sulfonamide in sediment

Total abundance (gene copies/g of sediment) of two genes conferring resistance to the antibiotics tetracycline (tet(W)) and sulfonamide (sul1) in sediment samples covering the period between 1920 and 2010 in Lake Geneva, Switzerland. Quantification was made in DNA extracted from the seed bank (SB DNA) and total microbial community (total DNA).

DOI: 10.7287/peerj.preprints.3361v1/supp-1

Representation of the overall bacterial seed bank community composition evaluated to the taxonomic level of Phylum

DOI: 10.7287/peerj.preprints.3361v1/supp-2

Relative abundance of the ten OTUs most correlated with the abundance of iron (top) and manganese (bottom) levels in the sediment samples used as proxies to lake eutrophication

DOI: 10.7287/peerj.preprints.3361v1/supp-3

Raw data to generate Fig 1 and Supplementary Fig 1

DOI: 10.7287/peerj.preprints.3361v1/supp-4

Additional Information

Competing Interests

The authors declare that they have no competing interest

Author Contributions

Laura Madueño performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Christophe Paul conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Thomas Junier performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Zhanna Bayrychenko performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Sevasti Filippidou performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Karin Beck performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Gilbert Greub conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper, historical data.

Helmut Bürgmann conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Pilar Junier conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

GenBank PRJNA396276

https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA396276

Funding

Swiss National Science Foundation (Interdisciplinary grant CR23I2_162810) for the funding of SF and TJ. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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