The first report of the prevalence of Nosema ceranae in Bulgaria

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1 Biodiversity, IBER-BAS, Sofia, Bulgaria
2 Department Pathology, Institute of Experimental Morphology, Pathology and Morphology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria;, Sofia, Bulgaria
3 Department of Structure and Function of Chromatin, Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria, Sofia, Bulgaria
4 Agricultural and Stockbreeding Experimental Station, Smolyan, Bulgaria, Agricultural Academy, Sofia, Bulgaria, Smolyan, Bulgaria
5 Laboratory of Agricultural Zoology and Entomology, Agricultural University of Athens, Athens, Greece
DOI
10.7287/peerj.preprints.3342v1
Published
Accepted
Subject Areas
Entomology, Genetics, Parasitology, Veterinary Medicine
Keywords
Apis mellifera, PCR diagnosis, Bulgaria, Nosematosis, Nosema spp. in Bulgaria
Copyright
© 2017 Hristov et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Hristov P, Georgieva A, Radoslavov G, Sirakova D, Dzhebir G, Shumkova R, Neov B, Bouga M. 2017. The first report of the prevalence of Nosema ceranae in Bulgaria. PeerJ Preprints 5:e3342v1

Abstract

Nosema apis and Nosema ceranae are the two main microsporidian parasites causing nosematosis in honey bee Apis mellifera. The object of the present study is to investigate the presence of Nosema apis and Nosema ceranae in the Bulgarian honey bee. The 16S (SSU) rDNA gene region was chosen for analysis. A duplex PCR assay was performed on 108 honey bee samples from three different parts of the country (South, North and West Bulgaria). The results showed that the samples from the northern part of the country were with the highest rate of invasion (77.2%) for Nosema ceranae while those from the mountainous parts (the Rodopa Mountains, South Bulgaria) were with the lowest rate (13.9%). Infection with Nosema apis alone and co-infection N. apis/N. ceranae were not detected in any samples. These findings suggest that Nosema ceranae is the dominant species in the Bulgarian honey bee. It is not known when the introduction of Nosema ceranae in Bulgaria has occurred, but like in the rest of the world, this species has become the dominant one in Bulgarian Apis mellifera. In conclusion, this is the first report for molecular detection of Nosema infection in Bulgarian honey bee that confirms the worldwide dissemination and prevalence of Nosema ceranae.

Author Comment

This is a submission to PeerJ for review.