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Supplemental Information

Alignment of COI gene sequences used in phylogenetic analyses

Identical sequences from different individuals were removed prior to analyses.

DOI: 10.7287/peerj.preprints.3332v1/supp-1

Alignment of COI gene sequences for all sequenced individuals

DOI: 10.7287/peerj.preprints.3332v1/supp-2

Alignment of NaK gene sequences for all sequenced individuals

DOI: 10.7287/peerj.preprints.3332v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Taylor M Greenan conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Charles L Griffiths conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, sampling.

Carlos A Santamaria conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, sampling.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Field collections were carried out under Scientific Collection Permit RES2017/53 issued by the South African Department of Environmental Affairs.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Sequences will be submitted to GenBank upon acceptance

Funding

Financial support for this project was provided by a National Research Foundation Incentive Grant to Charles L. Griffiths (#96043) as well as internal funding provided to Carlos A. Santamaria by the Department of Biological Sciences at Sam Houston State University and the College of Sciences and Mathematics at the University of South Florida Sarasota-Manatee. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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