Overexpression of VrPDF1 gene confers resistance to weevils in transgenic mung bean plants

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1 Department of Genetics & Modern Biology, School of Biology, Thainguyen University of Education, Thai Nguyen, Viet Nam
2 Department of Biotechnology, Bac Giang Agriculture and Forestry University, Bac Giang, Viet Nam
3 Lab. of Applied DNA Technology, Institute of Biotechnology, VAST, Ha Noi, Viet Nam
DOI
10.7287/peerj.preprints.3264v2
Published
Accepted
Subject Areas
Agricultural Science, Biotechnology, Genetics, Plant Science
Keywords
mung bean weevil, alpha-amylase inhibitor, Vigna radiata, transgenic mung bean, VrPDF1 gene
Copyright
© 2017 Ngoc Lan et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Ngoc Lan NT, Thao HT, Son LV, Hoang Mau C. 2017. Overexpression of VrPDF1 gene confers resistance to weevils in transgenic mung bean plants. PeerJ Preprints 5:e3264v2

Abstract

Plant defensins are known for different biological functions such as insect resistance, antibacterial, antifungal and antiviral activities. The role of plant defensins against weevils is based on inhibitor of alpha-amylase activity in weevil gut, therefore, weevils can not digest starch and then die. The low content of Vigna radiata plant defensin 1 (VrPDF1) in mung bean seeds is promted researches to increase the VrPDF1content, in which leads to enhance alpha-amylase inhibition and improve bruchid resistance in mung bean. This article presents the results of overexpression of VrPDF1 gene in transgenic mung bean plants. VrPDF1 gene was successfully transferred into DX22 mung bean cultivar and expressed in T1 generation transgenic mung bean seeds. The extract containing recombinant VrPDF1 protein inhibited alpha-amylase of weevil larvae in its larval stage. The performance of alpha-amylase inhibitor of DX1-3 and DX1-7 transgenic mung bean lines increased by 166.40% and 178.19% respectively, in comparison with non-transgenic plants. The enhancement of alpha-amylase inhibitor ability of rVrPDF1 extracted from transgenic plants is scientifically fundamental to confirm the effectiveness of the application of gene technology in enhancement the ability of mung bean weevil resistance in particular and grain crops in general revenue.

Author Comment

In this version the table and the figure are placed in its place in the article

Supplemental Information

Analysis of a-amylase activity of mung bean weevils (IU mg-) and Analysis of content of recombinant VrPDF1 protein by ELISA

DOI: 10.7287/peerj.preprints.3264v2/supp-1

Weevils were c ollected from mung bean warehouses in Bac Giang province

DOI: 10.7287/peerj.preprints.3264v2/supp-2