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Supplemental Information

Appendix S1 – DNA extraction details for the CLS extraction for arthropods

DOI: 10.7287/peerj.preprints.3184v1/supp-1

Appendix S2 – DNA extraction details for the modified bat guano extraction

DOI: 10.7287/peerj.preprints.3184v1/supp-2

Table S1 – Sequences for Ion barcoded primers

DOI: 10.7287/peerj.preprints.3184v1/supp-3

Figure S1 – Mitochondrial cytochrome oxidase C subunit 1 locus (CO1) primer map

DOI: 10.7287/peerj.preprints.3184v1/supp-4

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Michelle A Jusino conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Mark T Banik conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Jonathan M Palmer conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Amy K Wray performed the experiments, reviewed drafts of the paper.

Lei Xiao contributed reagents/materials/analysis tools.

Emma Pelton performed the experiments.

Jesse R Barber contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Akito Y Kawahara contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Claudio Gratton contributed reagents/materials/analysis tools, reviewed drafts of the paper.

M. Zachariah Peery contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Daniel L Lindner conceived and designed the experiments, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Boise State University Institutional Animal Care and Use Committee

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

All environmental guano samples were collected in accordance with Wisconsin Department of Natural Resources guidelines, and field protocols were approved by the Wisconsin Natural History Inventory (NHI) Program and the University of Wisconsin-Madison.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The corresponding data for this paper was deposited in the NCBI SRA (SRA study SRP102878; BioProject PRJNA380665), and barcoded primer information is provided in the supplemental information.

Data Deposition

The following information was supplied regarding data availability:

https://github.com/nextgenusfs/amptk

Funding

Funding for this work was provided by the US Forest Service, Northern Research Station and the Agricultural Experiment Station at the University of Wisconsin – Madison via Hatch Formula Funds, and NSF IOS-1121739 to AYK and IOS-1121807 to JRB. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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