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Virulence test using nematodes to prescreen Nocardia species capable of inducing neurodegeneration and behavioral disorders

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RT @uranus_2: ノカルディアという細菌がドーパミン系神経の変性を誘発する。パーキンソン病の一部は感染症によるかもしれないな。Virulence test using nematodes to prescreen Nocardia species …https://t…
ノカルディアという細菌がドーパミン系神経の変性を誘発する。パーキンソン病の一部は感染症によるかもしれないな。Virulence test using nematodes to prescreen Nocardia species …https://t.co/JzC1Hl4j3e
99 days ago
RT @fruitWormPapers: Virulence test using nematodes to prescreen Nocardia species capable of inducing neurodegeneration and behavioral… htt…
Virulence test using nematodes to prescreen Nocardia species capable of inducing neurodegeneration and behavioral… https://t.co/TgreKqfkOG
100 days ago
Virulence test using nematodes to prescreen Nocardia species capable of inducing neurodegeneration and behavioral… https://t.co/9Dqybpwpvl
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Supplemental Information

Mice infected by N. cyriacigeorgica GUH-2 presenting motor symptoms

Behavioral disorders in two mice. One with the rhythmic and vertical movements of the head (mouse up) and the other with hemiparesis (mouse down)

DOI: 10.7287/peerj.preprints.3152v1/supp-1

Number of nematodes affected in their movement using the touch response test

Wild type response to the touch response test is characterised by backward movements of the nematode and then a leak forward. We compared this reaction on N2 and BY250 nematodes lineages that were not in contact with supernatant (control). Nematode affected had the same reactions following the touching but with slow reactions or only backwards. We observed also forwards / backwards movements or motionless nematodes with only a movement of the nose. These kinds of phenotypes were observed on nematodes that were in contact with bacterial supernatant (N.farcinica 10152 or N.cyriacigeorgica GUH-2).

DOI: 10.7287/peerj.preprints.3152v1/supp-2

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Bernardin-Souibgui Claire performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Zoropogui Anthony performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Voisin Jeremy performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Ribun Sebastien performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Vasselon Valentin performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Pujic Petar conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Rodriguez-Nava Veronica contributed reagents/materials/analysis tools.

Belly Patrick performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, reviewed drafts of the paper.

Cournoyer Benoit reviewed drafts of the paper.

Didier Blaha conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All experiments were approved by the Institute of Higher Education in Food Science, Animal Health, Agricultural and Environmental Sciences, VetAgro Sup ethics committee.

Data Deposition

The following information was supplied regarding data availability:

We made Excell tables and these data are protected in internal web site without external access

Funding

This work was supported by Labex IMU (Project IMU-MIC) and ANR CABRRES. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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