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Supplemental Information

Figure 1a. Tumor weight after intravenous treatment

Complete tumor weight (in mg) after intravenous treatment (given in mg/kg bw) with BTB14431 and Emodin compared to the control group (isotone sodium chloride solution)

DOI: 10.7287/peerj.preprints.304v1/supp-1

Figure 1b. Tumor weight after intraperitoneal treatment

Entire tumor weight (in mg) following intraperitoneal treatment with BTB14431 and Emodin (given in mg/kg bw) compared to the control group (isotone sodium chloride solution)

DOI: 10.7287/peerj.preprints.304v1/supp-2

Figure 2a. Hemogram after intravenous therapy.

For identification of the groups see table 1 (Roman numerals, e.g. group one left two bars). Figure shows relative rate of leukocyte count (mean) in percent of 100%: pre op (0, left bar) and after last treatment (e.g. second bar). Neutrophil count increases whereas monocyte and lymphocyte count decreases during treatment. Eosinophil count increases in a lower rate.

DOI: 10.7287/peerj.preprints.304v1/supp-3

Figure 2b. Hemogram after intraperitoneal therapy.

Bar chart illustrates mean of leukocyte count in percent (compared to 100%). Neutrophil count increases while reduction of monocyte and lymphocyte count was observed at the end of treatment. Eosinophil count increases.

DOI: 10.7287/peerj.preprints.304v1/supp-4

Figure 3a. Apoptosis on granulocytes caused by Emodin

Intravenous application of Emodin is shown to induce apoptosis in differential blood sample (e.g. in pictogram 3 ‘nuclear fragmentation’ and in pictogram 11 ‘blebbing’ are seen; Magnitude x 10 for both)

DOI: 10.7287/peerj.preprints.304v1/supp-5

Figure 3b. Apoptotic effects on granulocytes by BTB 14431

Intravenous application of BTB14431 caused apoptosis (effects seen are comparable to Emodin – see fig. 3A; e.g. in pictogram no. 9 ‘nuclear fragmentation’ and in pictogram no. 20 ‘blebbing’ are seen)

DOI: 10.7287/peerj.preprints.304v1/supp-6

Table 1. Randomization into the different groups

Doses are given in mg per kg of the body weight (bw)

DOI: 10.7287/peerj.preprints.304v1/supp-7

Table 2. Time and reason of death during perioperative treatment

D1 to D7 – treatment at day 1 to 7, removal of port catheter system was performed at day 7; Reasons for death: Control group: port catheter related sepsis at day 6; bleeding after port removal; Group VII: during injection - cardiac and circulation arrest (CCA); Group XI: during injection – CCA; Group XII: during injection – CCA

DOI: 10.7287/peerj.preprints.304v1/supp-8

Table 3. Effects of treatment and application form on tumor load and body weight

For assignment of the groups see table 1. Ip-TM-weight: intraperitoneal tumor weight, sc-TM-weight: subcutaneous tumor weight; tumor load is given in gram (g) – median and range; bw: body weight; day 1: beginning of treatment; day 28: weight at autopsy; group V: only one animal evaluated, group VI all animals died

DOI: 10.7287/peerj.preprints.304v1/supp-9

Additional Information

Competing Interests

There are no competing interests.

Author Contributions

Stephan Rogalla conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Jana Pohlenz performed the experiments.

Vanessa Garcia analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Gerold Koplin performed the experiments.

Kirsten Fuehrer performed the experiments, observing rats.

Andreas Lun performed the experiments, contributed reagents/materials/analysis tools, prepared figures and/or tables.

Wolfgang Dubiel conceived and designed the experiments.

Caroline F. Geier performed the experiments.

Chris Braumann conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Approved by the local government committee of animal welfare:

1. Dr. H. Ratsch, LaGeSo Berlin, Postfach 31 09 29, 10639 Berlin

2. approval # G0192/06

Funding

This project was funded by internal funds of the University. No industrial or government fund was used. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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