Figure 1a. Tumor weight after intravenous treatment
Complete tumor weight (in mg) after intravenous treatment (given in mg/kg bw) with BTB14431 and Emodin compared to the control group (isotone sodium chloride solution)
Figure 1b. Tumor weight after intraperitoneal treatment
Entire tumor weight (in mg) following intraperitoneal treatment with BTB14431 and Emodin (given in mg/kg bw) compared to the control group (isotone sodium chloride solution)
Figure 2a. Hemogram after intravenous therapy.
For identification of the groups see table 1 (Roman numerals, e.g. group one left two bars). Figure shows relative rate of leukocyte count (mean) in percent of 100%: pre op (0, left bar) and after last treatment (e.g. second bar). Neutrophil count increases whereas monocyte and lymphocyte count decreases during treatment. Eosinophil count increases in a lower rate.
Figure 2b. Hemogram after intraperitoneal therapy.
Bar chart illustrates mean of leukocyte count in percent (compared to 100%). Neutrophil count increases while reduction of monocyte and lymphocyte count was observed at the end of treatment. Eosinophil count increases.
Figure 3a. Apoptosis on granulocytes caused by Emodin
Intravenous application of Emodin is shown to induce apoptosis in differential blood sample (e.g. in pictogram 3 ‘nuclear fragmentation’ and in pictogram 11 ‘blebbing’ are seen; Magnitude x 10 for both)
Figure 3b. Apoptotic effects on granulocytes by BTB 14431
Intravenous application of BTB14431 caused apoptosis (effects seen are comparable to Emodin – see fig. 3A; e.g. in pictogram no. 9 ‘nuclear fragmentation’ and in pictogram no. 20 ‘blebbing’ are seen)
Table 1. Randomization into the different groups
Doses are given in mg per kg of the body weight (bw)
Table 2. Time and reason of death during perioperative treatment
D1 to D7 – treatment at day 1 to 7, removal of port catheter system was performed at day 7; Reasons for death: Control group: port catheter related sepsis at day 6; bleeding after port removal; Group VII: during injection - cardiac and circulation arrest (CCA); Group XI: during injection – CCA; Group XII: during injection – CCA
Table 3. Effects of treatment and application form on tumor load and body weight
For assignment of the groups see table 1. Ip-TM-weight: intraperitoneal tumor weight, sc-TM-weight: subcutaneous tumor weight; tumor load is given in gram (g) – median and range; bw: body weight; day 1: beginning of treatment; day 28: weight at autopsy; group V: only one animal evaluated, group VI all animals died