Structure and properties of slow-resorbing nanofibers obtained by (co-axial) electrospinning as tissue scaffolds in regenerative medicine

, , , , , , , ,
1 Institute of Nonferrous Metals, Gliwice, Poland
2 Department of Pharmacological and Laboratory Medicine, Medical University of Silesia, Sosnowiec, Katowice
3 Department of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB, Canada
4 Health Policy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
5 Center for Biotechnology, Bioengineering and Bioinformatics, Silesian University of Technology, Gliwice, Poland
6 ENT, School of Medicine, Medical University of Silesia in Katowice, Katowice, Poland
7 Department of Anatomy, School of Health Science in Katowice, Medical University of Silesia, Katowice, Poland
8 Department of Pathology, Pomeranian Medical University, Szczecin, Poland
9 Institute of Metallurgy and Material Science Polish Academy of Science, Kraków, Poland
10 Małopolska Center of Biotechnology, Kraków, Poland
11 LInkocare Life Sciences AB, Linkoping, Sweden
DOI
10.7287/peerj.preprints.2971v1
Published
Accepted
Subject Areas
Bioengineering, Biotechnology, Synthetic Biology
Keywords
policaprolacotne, nanofibers, solution electrospinnning, core-shell nanofibers, co-axial electrospinning
Copyright
© 2017 Hudecki et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Hudecki A, Gola J, Ghavami S, Skonieczna M, Markowski J, Likus W, Lewandowska M, Maziarz W, Los MJ. 2017. Structure and properties of slow-resorbing nanofibers obtained by (co-axial) electrospinning as tissue scaffolds in regenerative medicine. PeerJ Preprints 5:e2971v1

Abstract

We investigated the structure and properties of PCL10 nanofiber, PCL5/PCL10 core-shell type nanofibers, as well as PCL5/PCLAg nanofibres prepared by electrospinning. For the production of the fibre variants, a 5-10% solution of polycaprolactone (Mw = 70000-90000), dissolved in a mixture of formic acid and acetic acid at a ratio of 70:30 m/m was used. In order to obtain fibres containing PCLAg 1% of silver nanoparticles was added. The electrospin was conducted using the above-described solutions at the electrostatic field. The subsequent bio-analysis shows that synthesis of core-shell nanofibers PCL5/PCL10, and the silver-doped variant nanofiber core shell PCL5/PCLAg by using organic acids as solvents is a robust technique. Such way obtained nanofibres may then be used in regenerative medicine for extracellular scaffolds: (i) for controlled bone regeneration due to the long decay time of the PCL, (ii) and as carriers of drug delivery nanocapsules. Furthermore, the used solvents are significantly less toxic than the solvents for polycaprolactone currently commonly used in electrospin, like for example chloroform (CHCl3), methanol (CH3OH), dimethylformamide (C3H7NO) or tetrahyfrofurna (C4H8O), hence the presented here electrospin technique may allow for the production of multilayer nanofibres more suitable for the use in medical field.

Author Comment

Regenerative medicine allows for in vitro-generation of artificial tissues and organs, hence answering the great shortfall in transplantation-medicine. One of the component of artificial tissues are artificial extracellular matrix (ECM) molecules, that for most applications should be resorbable so in the long term they could be replaced by own body materials. In the course of resorption, they may also serve as carriers supplying the tissues with growth-factors, antibiotics, or other therapeutics, that were previously embedded in their structures. We investigated the structure and properties of PCL10 nanofiber (the information in subscript supplements the information about material’s composition), PCL5/PCL10 core-shell type nanofibers, as well as PCL5/PCLAg nanofibres prepared by electrospinning. For the production of the fibre variants, a 5-10% solution of polycaprolactone (Mw = 70000-90000), dissolved in a mixture of formic acid and acetic acid at a ratio of 70:30 m/m was used. In order to obtain fibres containing PCLAg 1% of silver nanoparticles was added. The electrospin was conducted using the above-described solutions at the electrostatic field. Such way obtained nanofibres may then be used in regenerative medicine for extracellular scaffolds: (i) for controlled bone regeneration due to the long decay time of the PCL, (ii) and as carriers of drug delivery nanocapsules. Furthermore, the used solvents are significantly less toxic than the solvents for polycaprolactone currently commonly used in electrospin, like for example chloroform (CHCl3), methanol (CH3OH), dimethylformamide (C3H7NO) or tetrahyfrofurna (C4H8O), hence the presented here electrospin technique may allow for the production of multilayer nanofibres more suitable for the use in medical field.