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DNA-spiking in viral metagenome sequencing: A new method with low bias

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New preprint - DNA-spiking in viral metagenome sequencing: A new method with low bias https://t.co/E4b72yS56r #genomics https://t.co/HuGu3eMJmT
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Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Geert Cremers conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables.

Lavinia Gambelli performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Theo van Alen conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Laura van Niftrik analyzed the data, reviewed drafts of the paper.

Huub Op den Camp conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The raw sequence data were submitted to the European Nucleotide Archive and are available under accession number PRJEB20134. Direct link: (http://www.ebi.ac.uk/ena/data/view/PRJEB20134).

The viral genomes are available from GenBank under accession numbers, KX853510, KX853511, KX853512, KX853513, KX853514

Data Deposition

The following information was supplied regarding data availability:

European Nucleotide Archive

Accession number PRJEB20134

For direct access use:

http://www.ebi.ac.uk/ena/data/view/PRJEB20134

Funding

This work was supported by ERC Advanced grants (ERC-339880 and ERC-669371) and by the The Netherlands Organisation for Fundamental Research Gravitation grant (SIAM024002002). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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