Divergent evolutionary histories of DNA markers in a Hawaiian population of the coral Montipora capitata
- Subject Areas
- Ecology, Evolutionary Studies, Marine Biology
- genomics, sperm bundle DNA, coral colonies
- © 2017 Putnam et al.
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2017. Divergent evolutionary histories of DNA markers in a Hawaiian population of the coral Montipora capitata. PeerJ Preprints 5:e2896v1 https://doi.org/10.7287/peerj.preprints.2896v1
We investigated intra and inter-colony sequence variation in a population of the dominant Hawaiian coral Montipora capitata by analyzing marker gene and genomic data. Ribosomal ITS1 regions showed evidence of a reticulate history among the colonies, suggesting incomplete rDNA repeat homogenization. Analysis of the mitochondrial genome identified a major (M. capitata) and a minor (M. flabellata) haplotype in single polyp-derived sperm bundle DNA with some colonies containing 2-3 different mtDNA haplotypes. In contrast, Pax-C and newly identified single-copy nuclear genes showed either no sequence differences or minor variations in SNP frequencies segregating among the colonies. Our data suggest past mitochondrial introgression in M. capitata, whereas nuclear single-copy loci show limited variation, highlighting the divergent evolutionary histories of these coral DNA markers.
This is a submission to PeerJ for review.
Results of the NeighborNet analysis of the MTC data with the phi test result shown
The colony number demarcation and support values are the same as in Figure 2C.
Supplemental File 1
ITS alignment in fasta format
Supplemental File 2
MTC alignment in fasta format.
Supplemental File 3
Pax-C alignment in fasta format.