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Supplemental Information

Supplementary Table 1. Table containing data from Actinobacteria isolated from CCB

Sequence analysis of the cultured Actinobacteria isolated from the intermediate lagoon in Churince system, based on partial 16S rDNA gene sequencing.

DOI: 10.7287/peerj.preprints.2893v1/supp-1

Supplementary Figure 1. Complete phylogenetic reconstruction of phylum Actinobacteria based on 16S rDNA sequence

Phylum Actinobacteria Phylogenetic tree based on 16S rRNA sequences using Maximum Likelihood (ML) algorithm for representative CCB-isolated Actinobacteria and their closely related type strains. Bootstrap values based on 1000 replicates.

DOI: 10.7287/peerj.preprints.2893v1/supp-2

Supplementary Figure 2. NJ Phylogenetic tree for Complete Streptomycetaceae family

Phylogenetic tree based on a data set of 667 16S rRNA sequences of the Streptomycetaceae family using the neighbour-joining method. Bootstrap values based on 1000 replicates.

DOI: 10.7287/peerj.preprints.2893v1/supp-3

Data set for phylogenies

Complete data set used for phylogenetic analyses.

DOI: 10.7287/peerj.preprints.2893v1/supp-4

Additional Information

Competing Interests

Valeria Souza and Luis E. Eguiarte are Academic Editors for PeerJ.

Author Contributions

Hector Fernando Arocha-Garza performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables.

Ricardo Canales-Del Castillo analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, reviewed drafts of the paper.

Luis E. Eguiarte analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Valeria Souza conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Susana De la Torre-Zavala conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Access for sampling in Natural Protected Area in Cuatro Cienegas Coahuila was approved by Secretaría de Medio Ambiente y Recursos Naturales as stated by Permit: SGPA/DGVS/03121/15.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Supplemental File 3 contains DNA sequences reported in these experiments.

Data Deposition

The following information was supplied regarding data availability:

The raw data has been supplied as a supplementary file.

Funding

This research received funding from CONACYT (México) through Ciencias Básicas 2013 Project no. 221963 for Susana De la Torre Zavala. This research was also supported by Alianza WWF-Fundación Carlos Slim to Valeria Souza and Luis E. Eguiarte. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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