Comparative study of Cd uptake and tolerance of two Italian ryegrass (Lolium multiflorum) cultivars
- Published
- Accepted
- Subject Areas
- Plant Science, Toxicology
- Keywords
- Cadmium, Italian ryegrass, Physiological response, tolerance, uptake and translocation
- Copyright
- © 2017 Fang et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2017. Comparative study of Cd uptake and tolerance of two Italian ryegrass (Lolium multiflorum) cultivars. PeerJ Preprints 5:e2883v1 https://doi.org/10.7287/peerj.preprints.2883v1
Abstract
Cadmium (Cd) is one of the most toxic heavy metals and is difficult to remove from contaminated soil and water. Italian ryegrass (Lolium multiflorum), as an energy crop, exhibits a valuable potential to develop Cd polluted sites due to its use as a biofuel rather than as food and forage. Previously, via a screening for Cd-tolerant ryegrass, the two most extreme cultivars (IdyII and Harukaze) with high and low Cd tolerance during seed germination, respectively, were selected. However, the underlying mechanism for Cd tolerance was not well investigated. In this study, we comparatively investigated the growth, physiological responses, and Cd uptake and translocation of IdyII and Harukaze when the seedlings were exposed to a Cd (0-100 µM) solution for 12 days. As expected, excess Cd inhibited seedling growth and was accompanied by an accumulation of malondialdehyde (MDA) and reduced photosynthetic pigments in both cultivars. The effects of Cd on the uptake and translocation of other nutrient elements (Zn, Fe, Mn and Mg) were dependent on Cd concentrations, cultivars, plant tissues and elements. Compared with Harukaze, IdyII exhibited better performance with less MDA and higher pigment content. Furthermore, IdyII was less efficient in Cd uptake and translocation compared to Harukaze, which might be explained by the higher NPT (non-protein thiols) content in its roots. Taken together, our data indicate that IdyII is more tolerant than Harukaze, which partially resulted from the differences in Cd uptake and translocation.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
Data of plant biomass, Tls, root/shoot ratio were determined and the relation between Cd concentration and inhibition rate of root or shoot biomass were also determined
Raw data were applied for data analyse of biomass, Tls inhibition and preparation for Table1 and Table2
Cd uptake, the distribution proportion of Cd and Cd bioconcentration factors in root response to Cd
Nutrient elements uptake in root and shoot were determined under Cd stress
Raw data were applied for data analyses of metal uptake and preparation for Figure 3.
Pigment content of Italian ryegrass response to Cd
Raw data were applied for data analyses of correlation among root Tls, Cd uptake in root, TFs, MDA content of root, NPT content of root and preparation for Table 4.
The correlation among some physiological index were analysed
Raw data were applied for data analyses of correlation among root Tls, Cd uptake in root, TFs, MDA content of root, NPT content of root and preparation for Table 4.
MDA and NPT content of Italian ryegrass response to Cd
Raw data were applied for data analyses of MDA and NPT and preparation for Figure 4 and Figure 5.