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Supplemental Information

Unevenly chromosomal distribution of WRKY genes in wheat

Bar graphs indicated the number of TaWRKY genes in each chromosome, and broken line indicated the WRKY gene density per chromosome in wheat.

DOI: 10.7287/peerj.preprints.2809v1/supp-1

Gene structure of WRKY gene family in wheat

(A) The unrooted maximum-likelihood (ML) phylogenetic tree of TaWRKYs. The tree was constructed based on the WRKY domains from wheat by MEGA 7.0 with 1000 bootstrap. (B) Exon-intron composition of TaWRKY genes. (C) Intron number of each TaWRKY genes. See legends for detail information.

DOI: 10.7287/peerj.preprints.2809v1/supp-2

List of primers used for qRT-PCR experiments

DOI: 10.7287/peerj.preprints.2809v1/supp-3

The WRKY transcription factor family members in plants

DOI: 10.7287/peerj.preprints.2809v1/supp-4

Sequence features of WRKYs identified in wheat

DOI: 10.7287/peerj.preprints.2809v1/supp-5

The segmental and tandem duplication gene pairs identified in TaWRKYs.

DOI: 10.7287/peerj.preprints.2809v1/supp-6

Putative cis-acting elements identified in the promoter regions of TaWRKYs

DOI: 10.7287/peerj.preprints.2809v1/supp-7

The drought responsive AtWRKYs in in Arabidopsis and their putative orthologous TaWRKYs in wheat

DOI: 10.7287/peerj.preprints.2809v1/supp-8

Relative expression of TaWRKY genes in flag leaves, glumes and lemmas upon water deficit

DOI: 10.7287/peerj.preprints.2809v1/supp-9

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Pan Ning conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Congcong Liu conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables.

Jingquan Kang performed the experiments.

Jinyin Lv conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

The raw data has been supplied as a supplementary file.


This work was supported by the National Natural Science Foundation of China (No. 31271624). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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