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Supplemental Information

Gene Ontology classifications of assembled unigenes

The 27094 matched unigenes were classified into 3 functional categories: molecular function, biological process and cellular component.

DOI: 10.7287/peerj.preprints.2803v1/supp-1

Cluster analysis of 3844 differentially expressed genes during early stage of pollination

Un-pollinated, 30min, and 60 min means un-pollinated pistil, pollinated pistil after 30 min, and pollinated pistil after 60 min.

DOI: 10.7287/peerj.preprints.2803v1/supp-2

Supplemental table 1 The comparison of transcriptome data from two research group. Supplemental table 2 The information of 3844 differentially expressed genes

Supplemental table 1 The comparison of transcriptome data from two research group. Supplemental table 2 The information of 3844 differentially expressed genes.

DOI: 10.7287/peerj.preprints.2803v1/supp-3

Cluster analyses of differentially expressed genes which involved in the regulation of pollen tube growth process and double fertilization process during early stage of pollination

Un, 30, and 60 means un-pollinated pistil, pollinated pistil after 30 min, and pollinated pistil after 60 min. A means differentially expressed genes which involved in the regulation of pollen tube growth process and B means differentially expressed genes which involved in double fertilization process.

DOI: 10.7287/peerj.preprints.2803v1/supp-4

Supplementary Figure 1 Comparation of quality of sequencing data between our data and Nanjing’s data

a, b were Nanjing’s data and c, d were our data. The red and blue lines in a, c means median and average values of quality of sequencing data. The up line and down line of box plots in a, c means maximum and minimum values of quality of sequencing data. The red, blue, green and black lines in b, d means the concentration of T, C, A, and G.

DOI: 10.7287/peerj.preprints.2803v1/supp-5

Comparation of gene number between our data and Nanjing’s data

DOI: 10.7287/peerj.preprints.2803v1/supp-6

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Ming Li conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Kun Wang conceived and designed the experiments.

Rebecca Njeri Damaris reviewed drafts of the paper.

Pingfang Yang conceived and designed the experiments, analyzed the data, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The data reported here are deposited and publicly available at the National Center for Biotechnology Information (NCBI; https://www.ncbi.nlm.nih.gov/). Sequence raw data of Liriodendron chinense in fasta format (accession numbers SRR2039541, SRR2148838, SRR2148839, SRR2148840, SRR2148841, SRR2148842) are available at the NCBI.

Data Deposition

The following information was supplied regarding data availability:

The data reported here are deposited and publicly available at the National Center for Biotechnology Information (NCBI; https://www.ncbi.nlm.nih.gov/). Sequence raw data of Liriodendron chinense in fasta format (accession numbers SRR2039541, SRR2148838, SRR2148839, SRR2148840, SRR2148841, SRR2148842) are available at the NCBI.

Funding

This work was supported by the National Natural Science Foundation of China (No. No. 31300516). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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