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Supplemental Information

Characterization of ttg1-21 and ttg1-22

DOI: 10.7287/peerj.preprints.27974v1/supp-1

Alignment of TTG1, LWD1 and LWD2 CDS containing the primer binding sited for “TTG1 both” and “TTG1 no LWD” qRT-PCR experiments

DOI: 10.7287/peerj.preprints.27974v1/supp-2

Additional blots and analysis of protein levels in TTG1 overexpressor lines

DOI: 10.7287/peerj.preprints.27974v1/supp-3

Circadian profiles of endogenous and overexpressed TTG1 transcript levels

DOI: 10.7287/peerj.preprints.27974v1/supp-4

Similar to CO, GI transcript levels can not explain the significant reduction of FT transcript levels in TTG1 overexpression lines

DOI: 10.7287/peerj.preprints.27974v1/supp-5

Flowering time analysis of TTG1 gene regulatory network bHLH factor mutants and respective overexpression lines in A. thaliana Col-0 background at warm long day conditions

DOI: 10.7287/peerj.preprints.27974v1/supp-6

PRRs can re-localize TTG1 to different distinct (sub-)nuclear localizations and TTG1 can prevent the nuclear enrichment of CFP-bHLH92 as revealed by CLSM of nuclei in epidermal cells of infiltrated tobacco leaves

DOI: 10.7287/peerj.preprints.27974v1/supp-8

Epidermal areas of infiltrated tobacco leaves revealing the nuclear enrichment of RFP-TTG1 upon co-expression with PRRs and the abolished enrichment of CFP-bHLH92 by co-expression of RFP-TTG1

DOI: 10.7287/peerj.preprints.27974v1/supp-9

Datalogger results from growth condition measurements

DOI: 10.7287/peerj.preprints.27974v1/supp-10

Results of phenotyping experiments and statistics

DOI: 10.7287/peerj.preprints.27974v1/supp-11

Results of qRT-PCR experiments for circadian TTG1, CO, FT, SOC1 transcript levels in overexpression lines and statistics

DOI: 10.7287/peerj.preprints.27974v1/supp-12

Qualitative analysis of YFP-fluorescence in the overexpression lines as seen and classified by eye at a fluorescence stereo microscope

DOI: 10.7287/peerj.preprints.27974v1/supp-13

Results of circadian qRT-PCR experiments and statistics

DOI: 10.7287/peerj.preprints.27974v1/supp-14

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Barbara A M Paffendorf performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, approved the final draft.

Rawan Qassrawi performed the experiments, approved the final draft.

Andrea M Meys performed the experiments, approved the final draft.

Laura Trimborn performed the experiments, approved the final draft.

Andrea Schrader conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

Raw data are provided in supplementary files.


The authors received no funding for this work.

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