Development and characterization of novel cross-species tetranucleotide microsatellite markers for sterlet (Acipenser ruthenus) from Chinese sturgeon (Acipenser sinensis)

Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
DOI
10.7287/peerj.preprints.27964v1
Subject Areas
Aquaculture, Fisheries and Fish Science, Genetics
Keywords
Microsatellite markers, Cross-species, Acipenser ruthenus, Genetic diversity, Disomic, Tetrasomic
Copyright
© 2019 Hu et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Hu Y, Yang J, Liu X, Xiao K, Wang B, Du H. 2019. Development and characterization of novel cross-species tetranucleotide microsatellite markers for sterlet (Acipenser ruthenus) from Chinese sturgeon (Acipenser sinensis) PeerJ Preprints 7:e27964v1

Abstract

Sterlet (Acipenser ruthenus) is an important economic fish because of its nourishing caviar, isinglass and flesh. In order to facilitate the recovery of this species, the full understanding of its population genetic structure is necessary for taking appropriate management actions. However, genetic data on the use of nuclear loci in sterlet is still quite poor because microsatellite markers in sterlet that had been developed appeared to be polyploidy which add difficulties in studying the genetic of the sterlet. In this study, 24 tetranucleotide microsatellite markers were developed in sterlet from 160 microsatellite markers of the endangered Chinese sturgeon (Acipenser sinensis). Ten (ZHX76, ZHX64, Z194, Z217, Z184, Z242, Z250, Z258, Z268 and Z269) of the 24 loci showed disomic patterns while the rest loci showed tetrasomic patterns. In this paper, 24 microsatellite markers were characterized in 16 sterlet individuals and all of them were polymorphic with 2 to 7 alleles per locus. The Hardy-Weinberg departure value (d), polymorphic information content (PIC), the observed heterozygosity (HO), the Shannon-Wiener Diversity Indices (H') and the mean expected heterozygosity (HE) of all 24 polymorphic loci ranged from -0.334 to 0.484, 0.367 to 0.725, 0.438 to 1, 0.659 to 1.695, from 0.466 to 0.777, respectively. The markers described here will help in addressing practical problems such as the study of population genetics, conservation genetics and evolution in the polyploidy derivative nature of sterlet.

Author Comment

First, this work presents interesting results that microsatellite is conserved among closely related species and may be useful to clarify interspecific genetic relationships between recently diverged taxa. It has become fairly standard practice to test the newly developed microsatellite loci for cross-species utility. Chinese sturgeon and Sterlet sturgeon are closely relatives, and we first tried develop microsatellite for Sterlet sturgeon from Chinese sturgeon. Second, Ten of the 24 loci showed disomic pattern while the rest loci showed tetrasomic pattern. These novel tetranucleotide microsatellite markers will be useful tools to study genetic analyses and protection of Sterlet. In this study, the 24 novel microsatellite markers will be useful for the research of genetic management, genetic analysis and supportive breeding evaluation for Sterlet sturgeon.