The brown planthopper (BPH), Nilaparvata lugens, is the most destructive insect pest in rice production worldwide. The development and cultivation of BPH-resistant varieties is the most economical and efficient strategy to overcome the destruction caused by BPH. In this study, the modified bulk seedling test method was used to identify the BPH resistance level and host feeding choice of rice lines of Liangyou8676 (Bph14/Bph15), Bph68S (Bph14/Bph15), RHT (Bph3), Fuhui676, and TN1 on BPH. Meanwhile, the population, survival and emergence rate, developmental duration, honeydew excretion, female ratio and brachyptery ratio of adults were used as indicators to detect the antibiosis effects of the different rice lines. The results showed that the resistance levels of Rathu Heenati (RHT), Bph68S, Liangyou8676, Fuhui676, and TN1 to BPH were HR, R, MR, S and HS, respectively. The host choice implied that BPH was more inclined to feeding on rice plants with a lower resistance. An analysis of the antibiosis activity of rice lines RHT, BPh68S, and Liangyou8676 carrying resistance genes indicated a significant reduction in the population growth rate, survival and emergence rate of BPH nymphs, significant delay in the developmental duration of nymphs, reduced honeydew excretion of females, decreased female ratio, and a decreased brachyptery ratio of females and males, when compared with rice carrying no BPH-resistant genes.