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Background: In tumors, the role of human antigen R (HuR) involves regulating tumor cell proliferation, differentiation, apoptosis, angiogenesis, and lymphangiogenesis. Previous studies have revealed the expression of HuR can be detected in bladder cancer and related to biological behavior of malignancy. Methods: T24 cells were transfected by HuR overexpression vector and HuR knockdown vector. The cells were divided into control group, overExp-HuR group and cas9-HuR group. The cell viability after 48 h was detected by MTT, the apoptosis was detected by Annexin V-APC/7-AAD double staining, the cell migration was detected by Transwell assay, and the expression of HuR, cyclinD1 and apoptosis-related factors (Bcl-2) were detected by fluorescence quantitative PCR and Western blot. Results: Compared with the control group, the cell viability after 48 h in the overExp-HuR group increased significantly, and decreased in the cas9-HuR group (P<0.05). And the number of migrating cells increased in the overExp-HuR group, and decreased in the cas9-HuR group (P<0.05). The apoptosis rate of the overExp-HuR group decreased significantly, and increased significantly in the cas9-HuR group (P<0.05). The mRNA and protein expression of HuR, cyclinD1 and Bcl-2 of the overExp-HuR group increased, and decreased significantly in cas9-HuR group (P<0.05). Conclusion: HuR promote the proliferation and migration of T24 cells, and inhibit cell apoptosis. And the mechanism may be related to the expression of cyclin D and apoptosis-related proteins Bcl2.