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At the crossroads of botanical collections and molecular genetics laboratory: testing methods to obtain amplifiable DNA from moss herbarium material

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57 days ago
At the crossroads of botanical collections and molecular genetics laboratory: testing methods to obtain amplifiable DNA from moss herbarium material https://t.co/AvLD9RhWtY https://t.co/nnOFO8dCJp
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Supplemental Information

List of species tested within this study

DOI: 10.7287/peerj.preprints.27904v1/supp-1

Genetic regions and sequence primers used for testing DNA quality through both Qiagen and CTAB extraction tests

DOI: 10.7287/peerj.preprints.27904v1/supp-2

Qiagen extraction test. Genbank accession numbers for the studied specimens

DOI: 10.7287/peerj.preprints.27904v1/supp-3

DNA sequences generated with this study

DOI: 10.7287/peerj.preprints.27904v1/supp-4

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Marta Saługa conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The group of my sequences will be accessible via GenBank accession numbers:

ITS: MN179594 to MN179605

phy2 gene: MN239177 and MN239178

psbAF-trnHR2: MN239114 to MN239130

atpI-atpH: MN239131 - MN239140

trnL-trnF: MN239141 - MN239153

rps4 gene: MN239154 - MN239164

atpB1-rbcL1: MN239165 - MN239176

psbP-clpP: in preparation

Data Deposition

The following information was supplied regarding data availability:

All DNA sequences generated with this study are provided in supplementary file 1.

Funding

This study was financed by the National Science Centre, Poland, grant number: NCN 2015/17/B/N28/02475. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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