New mutations of env gene and its impact on virulence properties for Bovine leukemia virus
- Published
- Accepted
- Subject Areas
- Agricultural Science, Genetics, Genomics, Veterinary Medicine, Virology
- Keywords
- cattle leukemia, BLV, leucocytes, genotype, env gene, viral status
- Copyright
- © 2019 Blazhko et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2019. New mutations of env gene and its impact on virulence properties for Bovine leukemia virus. PeerJ Preprints 7:e27902v1 https://doi.org/10.7287/peerj.preprints.27902v1
Abstract
This study is the biodiversity and properties of bovine leukemia virus in Western Siberia. The researchers focused on exploring the polymorphism of the env gene and, in doing so, discovered the new genotypes Ia and Ib, which differ from genotype I. Restrictase Hae III sections the nucleotide sequence of the env gene intofragments with lengths of 316-27-95-5 bp (genotype I), 31-285-27-95-5 bp (genotype Ia), and 31-85-200-27-100 bp (genotype Ib). There are 2.57±0.55% (20 out of 779) samples of genotype Ib which does not differ significantly from 1% (χ2=2.46). Other genotypes were observed in the cattle of Siberia as wild type genotypes (their frequency varied from 17.84 to 32.73 %). This paper explores the effect of the env gene of the cattle leukemia virus on hematological parameters of infected animals. The maximum viral load was observed in animals with the II and IV viral genotypes (1000 – 1400 viral particles per 1000 healthy cells), and the minimum viral load was observed animals with genotype Ib (from 700 to 900 viral particles per 1000 healthy cells). Several hypotheses on the origin of the different genotypes in Siberia are discussed. The probability of the direct introduction of genotype II from South America to Siberia is extremely small and it is more likely that the strain originated independently in an autonomous population with its distribution also occurring independently. A new variety of genotype I (Ib) was found, which can be both a neoplasm and a relict strain.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
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