Tubular membranes extended between monolayer cells, from solid spheroids, and from clustered hollow spheroids in Ishikawa endometrial cell cultures can carry chromatin granules and mitonucleons
- Published
- Accepted
- Subject Areas
- Biochemistry, Cell Biology
- Keywords
- tubular membranes, unicellular hollow spheroids, multicellular hollow spheroids, mitonucleons, chromatin granules
- Copyright
- © 2019 Fleming
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2019. Tubular membranes extended between monolayer cells, from solid spheroids, and from clustered hollow spheroids in Ishikawa endometrial cell cultures can carry chromatin granules and mitonucleons. PeerJ Preprints 7:e27895v1 https://doi.org/10.7287/peerj.preprints.27895v1
Abstract
Membrane tubular extensions, recently recognized an important communication element in mammalian cells are demonstrated to form in Ishikawa endometrial epithelial cells growing in monolayers, and to extend from solid spheroids and from clustered hollow spheroids. Two kinds of chromatin cargoes travel through these tubules. Chromatin granules can pass through an endometrial tubule bridge extending from one monolayer fragment to another. The passage of granules over time from one of the fragments appears to support the self-assembly of nuclei in the other colony fragment. Similarly, in a process detected by observing an open-ended membrane tubule extending from a solid cell spheroid, a nucleus was observed to form over a period of 3 hours. Indications are that chromatin granules such as those observed in the amitotic processes of epithelial dome cell formation and of hollow spheroid cell formation are contributing to the build up of nuclei. Mitonucleons, a transient subcellular organelle consisting of fused mitochondria intimately associated with aggregated chromatin are also observed to pass through tubular membrane extensions. Multiple membrane extensions can be shown to to extend from clusters of unicellular polyploid hollow spheroids whose formation is described for the first time in this paper.
Author Comment
This is a preprint submission to PeerJ Preprints