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Supplemental Information

Detection of Male Ploidy

It shows the results of ploidy detection of male wasps (primary indicator) in "Detection of Diploid Males" part by a qPCR method.

DOI: 10.7287/peerj.preprints.27871v1/supp-1

Inbreeding&Outcross

It shows the results of multiple generations of consecutive inbreeding and outcross (control), whereby brood size, offspring sex ratio and offspring mortality (secondary indicators) can be calculated and analyzed.

DOI: 10.7287/peerj.preprints.27871v1/supp-2

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Quan-quan Liu conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Jin-cheng Zhou conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Chen Zhang performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables.

Qian-jin Dong performed the experiments, analyzed the data.

Su-fang Ning performed the experiments, analyzed the data.

Dong Hui conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

The raw measurements are provided in the supplementary file 1 and 2.

The raw data in file 1 shows the results of ploidy detection of male wasps (primary indicator) in "Detection of Diploid Males" part by a qPCR method.

File 2 shows the results of multiple generations of consecutive inbreeding and outcross (control), whereby brood size, offspring sex ratio and offspring mortality (secondary indicators) can be calculated and analyzed.

Funding

This work was supported by the National Key Research and Development Program of China (2017YFD0201000). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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