Callus induction of tartary buckwheat and enhancement of its flavonoids via FtCHS1 overexpression
- Published
- Accepted
- Subject Areas
- Molecular Biology, Plant Science
- Keywords
- Callus, Tartary buckwheat, Chalcone synthase, Flavonoids, tartary buckwheat
- Copyright
- © 2019 Dong et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2019. Callus induction of tartary buckwheat and enhancement of its flavonoids via FtCHS1 overexpression. PeerJ Preprints 7:e27862v1 https://doi.org/10.7287/peerj.preprints.27862v1
Abstract
Tartary buckwheat (Fagopyrum tataricum), a popular and traditional health care-related cereal, has recently been the focus of research because of its metabolic regulation of flavonoids. Elicitingtissues in vitroculture is an effective way to explore flavonoid biosynthesis mechanisms in tartary buckwheat. In the present study, we developed an in vitro genetic transformation system using the tartary buckwheat variety ‘Xiqiao No. 2’. The results showed thattherate of callus induced from hypocotylexplants on Murashige and Skoog (MS) medium containing 0.8 mg/L 6-BA and 3.5 mg/L 2,4-D was 100%. Much greater amounts of calli could then be obtained by repeated subculture on MS medium supplemented with 3.0 mg/L 6-BA and 1.0 mg/L KT. Furthermore, transgenic calli expressing the FtCHS1 gene were obtained viaAgrobacterium-mediatedtransformation. Overexpressing FtCHS1 in tartary buckwheat callus led tothe marked promotion of flavonol (P<0.01) and anthocyanin accumulation (P<0.05) due to the dramatic upregulation of the transcription of FtCHI, FtCHS2, FtFLS1, FtFLS2, FtFLS3 and FtDFR1, the genes of key enzymes involved in the flavonol and anthocyanin biosynthesis pathways (P < 0.01). This study provides solid support for further transgenic manipulation of calli as part of a system for regenerating tartary buckwheat.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
Table S1 .The primer sequence table
Sequence of primers