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Improved salt tolerance in transgenic tobacco by over-expression of poplar NAC13 gene

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NAC13-over-expressing tobacco https://t.co/CHeuqSEPa2 via @PeerJPreprints
PeerJ: Improved salt tolerance in transgenic tobacco by over-expression of poplar NAC13 gene https://t.co/dhd2UXNB5b
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Supplemental Information

Prime names and sequences

The primers of NAC13 are used to amplify the coding sequence of NAC13 gene. NAC13F1 and NAC13R1 are used to clone the ORF sequence of NAC13 gene; NAC13F2 and NAC13R2 are used to generate the recombinant construct 35S::NAC13-GFP, and the underlined area is the restriction site Spe I; NAC13F3 and NAC13R3, NAC13aF and NAC13aR, NAC13bF and NAC13bR are used to generate bait vector pGBKT7-NAC13, pGBKT7-NAC13a and pGBKT7-NAC13b, respectively. The underlined areas are EcoR I and Sal I restriction sites; NAC13F4 and NAC13R4 are used to amplify the complete cDNA sequence of NAC13 gene and introduce into the plant binary vector pBI121. The underlined areas are restriction sites Xba I and Sac I.

DOI: 10.7287/peerj.preprints.27861v1/supp-1

The characteristics of NAC13 gene in 84K poplar

Supplemental Figure 1. The characteristics of NAC13 gene in 84K poplar. (A) Gene structure of NAC13 as predicted by Gene Structure Display Sever; (B) Phylogenetic tree analysis of NACs from different plants by MEGA 5.1 using Neighbor-Joining method; (C) Alignment of amino acid sequences of NACs by Clustal W.

DOI: 10.7287/peerj.preprints.27861v1/supp-2

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Xuemei Zhang conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, approved the final draft.

Zihan Cheng performed the experiments, analyzed the data, approved the final draft.

Kai Zhao performed the experiments, approved the final draft.

Renhua Li analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Boru Zhou approved the final draft.

Tingbo Jiang contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

The raw measurements are provided in the supplementary Figure 1.

Funding

This work was supported by the National Key Program on Transgenic Research (2018ZX08020002) and the 111 project (B16010). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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