Prime names and sequences
The primers of NAC13 are used to amplify the coding sequence of NAC13 gene. NAC13F1 and NAC13R1 are used to clone the ORF sequence of NAC13 gene; NAC13F2 and NAC13R2 are used to generate the recombinant construct 35S::NAC13-GFP, and the underlined area is the restriction site Spe I; NAC13F3 and NAC13R3, NAC13aF and NAC13aR, NAC13bF and NAC13bR are used to generate bait vector pGBKT7-NAC13, pGBKT7-NAC13a and pGBKT7-NAC13b, respectively. The underlined areas are EcoR I and Sal I restriction sites; NAC13F4 and NAC13R4 are used to amplify the complete cDNA sequence of NAC13 gene and introduce into the plant binary vector pBI121. The underlined areas are restriction sites Xba I and Sac I.
The characteristics of NAC13 gene in 84K poplar
Supplemental Figure 1. The characteristics of NAC13 gene in 84K poplar. (A) Gene structure of NAC13 as predicted by Gene Structure Display Sever; (B) Phylogenetic tree analysis of NACs from different plants by MEGA 5.1 using Neighbor-Joining method; (C) Alignment of amino acid sequences of NACs by Clustal W.