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Analysis of genetic diversity in the coastal and island endangered plant Elaeagnus macrophylla by conserved DNA-derived polymorphism markers

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Analysis of genetic diversity in the coastal and island endangered plant Elaeagnus macrophylla by conserved DNA-derived polymorphism markers https://t.co/HQPI5imCKp https://t.co/BLXJXS3CFN
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Supplemental Information

A 0-1 matrix of 15 primer-pair amplified 102 samples

0-1 matrix obtained from the results of electropherogram.

DOI: 10.7287/peerj.preprints.27850v1/supp-1

Screening of primers ERF2, ERF3, KNOX-1 by 7 samples

It can be seen that the three primer amplification bands are clear and specific and can be used for experiments.

DOI: 10.7287/peerj.preprints.27850v1/supp-2

Screening of primers MYB1, MYB2, ERF1 by 7 samples

It can be seen that the MYB1, MYB2, ERF1 amplified bands are clear and specific and can be used for experiments.

DOI: 10.7287/peerj.preprints.27850v1/supp-3

Screening of primers WRKY-F1, WRKY-R1, WRKY-R2 by 7 samples

It can be seen that the WRKY-F1, WRKY-R1 amplified bands are clear and specific and can be used for experiments. Although the WRKY-R2 has a strip, the strip is weak and therefore not used.

DOI: 10.7287/peerj.preprints.27850v1/supp-4

Screening of primers WRKY-R3, WRKY-R2B, WRKY-R3B by 7 samples

It can be seen that the WRKY-R3 amplified band is clear and specific, and can be used for experiments. WRKY-R2B, WRKY-R3B is not effective, so it is not used.

DOI: 10.7287/peerj.preprints.27850v1/supp-5

Screening of primers KNOX-2, KNOX-3, MADS-1 by 7 samples

It can be seen that KNOX-2, KNOX-3, MADS-1 amplified bands are clear and specific and can be used for experiments.

DOI: 10.7287/peerj.preprints.27850v1/supp-6

Screening of primers ABP1-1, ABP1-2, ABP1-3 by 7 samples

It can be seen that the ABP1-1, ABP1-3 amplified bands are clear and specific and can be used for experiments. ABP1-2 did not amplify the band and therefore could not be used.

DOI: 10.7287/peerj.preprints.27850v1/supp-7

Screening of primers MADS-2, MADS-3, MADS-4 by 7 samples

It can be seen that the MADS-4 amplification band is clear and specific and can be used for experiments. Although the MADS-2 has a band, it is not effective in use, and the MADS-3 does not amplify the band, so it is not used.

DOI: 10.7287/peerj.preprints.27850v1/supp-8

LGD1-8,DRS9-20,WRKY-F1

Amplification results of WRKY-F1 on LGD1-8 and DRS9-20.

DOI: 10.7287/peerj.preprints.27850v1/supp-9

DRS21-28,NJD1-12

Amplification results of WRKY-F1 on DRS21-28, NJD1-12.

DOI: 10.7287/peerj.preprints.27850v1/supp-10

NJD13-NJD32,WRKY-F1

Amplification results of WRKY-F1 on NJD13-NJD32.

DOI: 10.7287/peerj.preprints.27850v1/supp-11

NJD 33,LS 1-13,PTD1,LSD1,WRKF-F1

Amplification results of WRKY-F1 on NJD 33, LS 1-13, PTD1, LSD1.

DOI: 10.7287/peerj.preprints.27850v1/supp-12

DGD1-13,WRKY-F1

Amplification results of WRKY-F1 on DGD1-13.

DOI: 10.7287/peerj.preprints.27850v1/supp-13

DGD14-26,WRKY-F1

Amplification results of WRKY-F1 on DGD14-26.

DOI: 10.7287/peerj.preprints.27850v1/supp-14

LGD1-8,DRS9-20,WRKY-R1

Amplification results of WRKY-R1 on LGD1-8 and DRS9-20.

DOI: 10.7287/peerj.preprints.27850v1/supp-15

DRS21-28,NJD1-12,WRKY-R1

Amplification results of WRKY-R1 on DRS21-28, NJD1-12.

DOI: 10.7287/peerj.preprints.27850v1/supp-16

NJD13-32,WRKY-R1

Amplification results of WRKY-R1 on NJD13-32.

DOI: 10.7287/peerj.preprints.27850v1/supp-17

NJD 33,LS1-13,PTD1,LSD1,WRKY-R1

Amplification results of WRKY-R1 on NJD 33, LS1-13, PTD1, LSD1.

DOI: 10.7287/peerj.preprints.27850v1/supp-18

DGD1-20,WRKY-R1

Amplification results of WRKY-R1 on DGD1-20.

DOI: 10.7287/peerj.preprints.27850v1/supp-19

DGD21-26,WRKY-R1

Amplification results of WRKY-R1 on DGD21-26.

DOI: 10.7287/peerj.preprints.27850v1/supp-20

LGD1-8,DRS9-20,WRKY-R3

Amplification results of WRKY-R3 on LGD1-8, DRS9-20 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-21

DRS21-28,NJD1-12,WRKY-R3

Amplification results of WRKY-R3 on DRS21-28, NJD1-12 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-22

NJD13-32,WRKY-R3

Amplification results of WRKY-R3 on DRS21-28, NJD13-32 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-23

NJD33,LS1-13,PTD1,LSD1,WRKY-R3

Amplification results of WRKY-R3 on NJD33, LS1-13, PTD1, LSD1 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-24

DGD1-20,WRKY-R3

Amplification results of WRKY-R3 on DGD1-20 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-25

DGD21-26,WRKY-R3

Amplification results of WRKY-R3 on DGD21-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-26

LGD1-8,DRS9-20,MYB1

Amplification results of MYB1 on LGD1-8, DRS9-20 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-27

DRS21-28,NJD1-12,MYB1

Amplification results of MYB1 on DRS21-28, NJD1-12 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-28

NJD13-33,MYB1

Amplification results of MYB1 on NJD13-33 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-29

NJD33,LS1-13,PTD1,LSD1,MYB1

Amplification results of MYB1 on NJD33, LS1-13, PTD1, LSD1 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-30

DGD1-20,MYB1

Amplification results of MYB1 on DGD1-20 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-31

DGD21-26,MYB1

Amplification results of MYB1 on DGD21-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-32

LGD1-8,DRS9-20,MYB2

Amplification results of MYB2 on LGD1-8, DRS9-20 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-33

DRS21-28,NJD1-12,MYB2

Amplification results of MYB2 on DRS21-28, NJD1-12 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-34

NJD13-32,MYB2

Amplification results of MYB2 on NJD13-32 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-35

NJD33,LS1-13,PUD1,LSD1,MYB2

Amplification results of MYB2 on NJD33, LS1-13, PUD1, LSD1 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-36

DGD1-20,MYB2

Amplification results of MYB2 on DGD1-20 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-37

DGD21-26,MYB2

Amplification results of MYB2 on DGD21-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-38

LGD1-8,DRS9-20,ERF1

Amplification results of ERF1 on LGD1-8, DRS9-20 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-39

DRS21-28,NJD1-12,ERF1

Amplification results of ERF1 on DRS21-28, NJD1-12 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-40

NJD13-32,ERF1

Amplification results of ERF1 on NJD13-32 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-41

NJD33,LS1-13,PTD1,LSD1,ERF1

Amplification results of ERF1 on NJD33, LS1-13, PTD1, LSD1 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-42

DGD1-20,ERF1

Amplification results of ERF1 on DGD1-20 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-43

LS1-6,DGD21-26,ERF1

Amplification results of ERF1 on LS1-6, DGD21-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-44

LGD1-8,DRS9-20,ERF2

Amplification results of ERF2 on LGD1-8, DRS9-20 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-45

DRS21-28,NJD1-12,ERF2

Amplification results of ERF2 on DRS21-28, NJD1-12 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-46

NJD13-32,ERF2

Amplification results of ERF2 on NJD13-32 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-47

NJD33,LS1-13,PTD1,LSD1,ERF2

Amplification results of ERF2 on NJD33, LS1-13, PTD1, and LSD1 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-48

DGD1-20,ERF2

Amplification results of ERF2 on DGD1-20 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-49

DGD21-26,ERF2

Amplification results of ERF2 on DGD21-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-50

LGD1-8,DRS9-20,ERF3

Amplification results of ERF3 on LGD1-8, DRS9-20 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-51

DRS21-28,NJD1-12,ERF3

Amplification results of ERF3 on DRS21-28, NJD1-12 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-52

NJD13-32,ERF3

Amplification results of ERF3 on NJD13-32 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-53

NJD33,LS1-13,PTU1,LSD1,DGD24-26,ERF3

Amplification results of ERF3 on NJD33, LS1-13, PTU1, LSD1, and DGD24-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-54

DGD1-23,ERF3

Amplification results of ERF3 on DGD1-23 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-55

DGD24-26,ERF3

Amplification results of ERF3 on DGD24-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-56

LGD1-8,DRS9-20,KNOX-1

KNOX-1 amplification results for LGD1-8, DRS9-20 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-57

DRS21-28,NJD1-12,KNOX-1

KNOX-1 amplification results for DRS21-28, NJD1-12 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-58

NJD13-32,KNOX-1

KNOX-1 amplification results for NJD13-32 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-59

NJD33,LS1-13,PUD1,LSD1,KNOX-1

KNOX-1 amplification results for NJD33, LS1-13, PUD1, LSD1 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-60

DGD1-20,KNOX-1

KNOX-1 amplification results for DGD1-20 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-61

DGD21-26,KNOX-1

KNOX-1 amplification results for DGD21-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-62

LGD1-8,DRS9-23,KNOX-2

KNOX-2 amplification results for LGD1-8, DRS9-23 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-63

DRS24-28,NJD1-18,KNOX-2

KNOX-2 amplification results for DRS24-28, NJD1-18 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-64

NJD19-33,LS1-6,KNOX-2

KNOX-2 amplification results for NJD19-33, LS1-6 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-65

LS7-13,PTD1,LSD1,DGD1-13,KNOX-2

KNOX-2 amplification results for LS7-13, PTD1, LSD1and DGD1-13 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-66

DGD14-26,KNOX-2

KNOX-2 amplification results for DGD14-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-67

LGD1-8,DRS9-23,KNOX-3

KNOX-3 amplification results for LGD1-8, DRS9-23 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-68

DRS24-28,NJD1-18,KNOX-3

KNOX-3 amplification results for DRS24-28, NJD1-18 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-69

NJD19-33,PTD1,LSD1,LS1-4,KNOX-3

KNOX-3 amplification results for NJD19-33, PTD1, LSD1, LS1-4 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-70

LS5-13,DGD1-13,KNOX-3

KNOX-3 amplification results for LS5-13, DGD1-13 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-71

DGD14-26,KNOX-3

KNOX-3 amplification results for DGD14-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-72

LGD1-8,DRS9-23,MADS-1

Amplification results of MADS-1 on LGD1-8, DRS9-23 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-73

DRS24-28,NJD1-18,MADS-1

Amplification results of MADS-1 on DRS24-28, NJD1-18 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-74

NJD19-NJD33,PTD1,LSD1,LS1-4,MADS-1

Amplification results of MADS-1 on NJD19-NJD33, PTD1, LSD1, LS1-4 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-75

LS5-13,DGD1-13,MADS-1

Amplification results of MADS-1 on LS5-13, DGD1-13 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-76

DGD14-26,MADS-1

Amplification results of MADS-1 on DGD14-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-77

LGD1-8,DRS9-23,MADS-4

Amplification results of MADS-4 on LGD1-8 and DRS9-23 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-78

DRS24-28,NJD1-18,MADS-4

Amplification results of MADS-4 on DRS24-28, NJD1-18 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-79

NJD19-33,PUD1,LSD1,LS1-4,MADS-4

Amplification results of MADS-4 on NJD19-33, PUD1, LSD1, LS1-4 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-80

LS5-13,DGD1-13,MADS-4

Amplification results of MADS-4 on LS5-13, DGD1-13 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-81

DGD14-26,MADS-4

Amplification results of MADS-4 on DGD14-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-82

LGD1-8,DRS9-23,ABP1-1

ABP1-1 amplification results for LGD1-8, DRS9-23 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-83

DRS24-28,NJD1-18,ABP1-1

ABP1-1 amplification results for DRS24-28, NJD1-18 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-84

NJD19-33,LS1-4,ABP1-1

ABP1-1 amplification results for NJD19-33, LS1-4 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-85

LS5-13,DGD1-13,ABP1-1

ABP1-1 amplification results for LS5-13, DGD1-13 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-86

DGD14-26,ABP1-1

ABP1-1 amplification results for DGD14-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-87

LGD1-8,DRS9-23,ABP1-3

ABP1-3 amplification results for LGD1-8, DRS9-23 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-88

DRS24-28,NJD1-18,ABP1-3

ABP1-3 amplification results of DRS24-28, NJD1-18 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-89

NJD19-33,PTD1,LSD1,LS1-4,ABP1-3

ABP1-3 amplification results for NJD19-33, PTD1, LSD1, LS1-4 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-90

LS5-13,DGD1-13,ABP1-3

ABP1-3 amplification results for LS5-13, DGD1-13 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-91

DGD14-26,ABP1-3

ABP1-3 amplification results for DGD14-26 samples.

DOI: 10.7287/peerj.preprints.27850v1/supp-92

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Wang Yi conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Yan Ma conceived and designed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper.

Bingyu Jia performed the experiments, prepared figures and/or tables.

Qichao Wu collection of experimental samples.

Dekui Zang contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper.

Xiaoyan Yu conceived and designed the experiments, contributed reagents/materials/analysis tools.

Data Deposition

The following information was supplied regarding data availability:

The raw data is as follows:Table 1 contains the 0-1 matrix of the amplified results of the 15 primers for 102 samples. Among them, Liugong Island population (1-8), Da Rushan population (9-28), Nanji Island population (J1-J33), Putuo island sample (Putuo), Lingshan island sample (LINGSHAN), Laoshan population (L1-L13), Daguan Island Population (DGD1-DGD26). Picture 2 -

Funding

This study was funded by the project of Shandong Provincial Agricultural Elite Varieties Project (2016LZGC038) and Forestry Science & Technology Innovation Project of Shandong Province (LYCX01-2018-03) , China; Shandong Agricultural Seeds Engineering Project (Collection, Protection and Precision Identification of Forest Tree Germplasm Resources). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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