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Oomycete metabarcoding reveals the presence of Lagenidium spp. in phytotelmata

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333 days ago
My preprint has been published today in @PeerJPreprints https://t.co/dsiQYWQH9u #Biodiversity #Genomics #Microbiology #MolecularBiology
333 days ago
PJpreprint: Oomycete metabarcoding reveals the presence of Lagenidium spp. in phytotelmata https://t.co/67sfqQMgBa
Oomycete metabarcoding reveals the presence of Lagenidium spp. in phytotelmata https://t.co/ofd21BB7k2 https://t.co/RGLnfYN5cU
Oomycete metabarcoding reveals the presence of Lagenidium spp. in phytotelmata https://t.co/LclpCVeBTY https://t.co/FdnuPCWgyn
Oomycete metabarcoding reveals the presence of Lagenidium spp. in phytotelmata https://t.co/63fuhnGvE6 https://t.co/8BNTtM1zbk
Oomycete metabarcoding reveals the presence of Lagenidium spp. in phytotelmata https://t.co/xjToAhhF3X https://t.co/MPo47EQ6VK
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Supplemental Information

Raw sequences associated with the GenBank accession numbers MN099105-MN099125

All sequences were submitted to GenBank on July 24 with no specific release date. They should be available shortly using the assigned accession numbers- once they have been fully processed by the GenBank staff.

DOI: 10.7287/peerj.preprints.27835v1/supp-1

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Paula Leoro-Garzon performed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Andrew J Gonedes performed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Isabel E Olivera performed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Aurelien Tartar conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The sequence corresponding to the L. giganteum cox1 gene is available publicly in the GenBank/EMBL/ DDBJ databases under the accession number MN099105.

Similarly, plant barcodes generated in this study were deposited under the accession numbers MN099106-MN099113, while oomycete cox1 barcodes produced using the gene cloning/Sanger sequencing strategies are accessible with the accession numbers MN099114-MN099125.

The sequences reads corresponding to cox1 barcodes generated using high-throughput sequencing (PacBio) technologies are available in the NCBI Sequence Read Archive (SAR) data under accession numbers SRX6359420, SRX6359421, SRX6359422, SRX6359423 (plants P1-P4, respectively) as part of Bioproject PRJNA550619

Data Deposition

The following information was supplied regarding data availability:

The raw data correspond to the sequences that were all deposited in appropriate, public databases

Funding

Funding for this project was provided through grants from the US Department of Agriculture (Agriculture and Food Research Initiative 2011-68004-30104) and a US Department of Education (Minority Science and Engineer Improvement Program P120A140012). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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