Genome-wide characterization and expression analysis of the Dof gene family related to abiotic stress in watermelon

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1 Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, Jiangxi, China
2 College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi province, China
3 Zhejiang Academy of Agricultural Sciences, State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Hanghzou, Zhejiang, China
4 Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Collaborative Innovation Center of Post-Harvest Key Technology and Quality Safety of Fruits and Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang, Jiangxi, China
5 Pingxiang University, Pingxiang, Jiangxi province, China
DOI
10.7287/peerj.preprints.27819v1
Published
Accepted
Subject Areas
Agricultural Science, Genomics
Keywords
Watermelon, Dof, Phylogenetic analysis, Expression profile, Abiotic stress
Copyright
© 2019 Zhou et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Zhou Y, Cheng Y, Wan C, Yang Y, Chen J. 2019. Genome-wide characterization and expression analysis of the Dof gene family related to abiotic stress in watermelon. PeerJ Preprints 7:e27819v1

Abstract

The plant DNA-binding with one finger (Dof) gene family is a class of plant-specific transcription factors that play vital roles in many biological processes and response to stresses. In the present study, a total of 36 ClDof genes were identified in the watermelon genome, which were unevenly distributed on 10 chromosomes. Phylogenetic analysis showed that the ClDof proteins could be divided into nine groups, and the members in a particular group had similar motif arrangement and exon-intron structure. We then analyzed the expression patterns of nine selected ClDof genes in eight specific tissues by qRT-PCR, and the results showed that they have tissue-specific expression patterns. We also evaluated the expression levels of the nine selected ClDof genes under salt stress and ABA treatments using qRT-PCR, and they showed differential expression under these treatments, suggesting their important roles in stress response. Taken together, our results provide a basis for future research on the biological functions of Dof genes in watermelon.

Author Comment

This is a submission to PeerJ for review.

Supplemental Information

Table S1

Primers sequences used in qRT-PCR.

DOI: 10.7287/peerj.preprints.27819v1/supp-1

Table S2. The qRT-PCR results of the expression profiles of nine selected ClDof genes in various tissues, under salt and ABA treatment

DOI: 10.7287/peerj.preprints.27819v1/supp-2