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Effect of dietary concentrate to forage ratio on growth performance, rumen fermentation and bacterial diversity of Tibetan sheep under barn feeding on Qinghai-Tibetan plateau

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Effect of dietary concentrate to forage ratio on growth performance, rumen fermentation and bacterial diversity of Tibetan sheep under barn feeding on Qinghai-Tibetan plateau https://t.co/SwmszY8Bhf https://t.co/6WhwUG49Oj
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Supplemental Information

The raw data of the growth performance of Tibetan sheep

The treatment HS1= concentrate to forage ratio 0:100, HS2= concentrate to forage ratio 15:85, HS3= concentrate to forage ratio 30:70, HS4= concentrate to forage ratio 45:55, HS5=concentrate to forage ratio 60:40. DMI=dry matter intake; DM consume= DMI×t, t is experimental time(d), BW=body weight; ADG= average daily BW gain . FCR= feed conversion ratio.

DOI: 10.7287/peerj.preprints.27807v2/supp-1

The supplementary materials of the manuscript

Table S1 The nutrition of concentrate and forage feed in the experiment. Table S2 The statistics of raw rate across the 20 different samples. Table S3 The relative abundance of the total phyla detected by taxonomic analysis (%). Table S4 The relative abundance of total genus detected by taxonomic analysis (%). Table S5 The relative abundance of the 8 most dominant bacteria at the phylum level. Table S6 The effect of increases dietary concentrate on the rumen bacteria at the genus level.

DOI: 10.7287/peerj.preprints.27807v2/supp-2

Clean data of Tibetan sheep in group HS1

The 2 sequences in the file are represented 2 samples of Tibetan sheep in group HS1.

DOI: 10.7287/peerj.preprints.27807v2/supp-3

Clean data of Tibetansheep in group HS1

The 2 sequences in the file are represented another 2 samples of Tibetan sheep in group HS1.

DOI: 10.7287/peerj.preprints.27807v2/supp-4

Clean data of Tibetan sheep in group HS2

The 4 sequences in the file are represented 4 samples of Tibetan sheep in group HS2.

DOI: 10.7287/peerj.preprints.27807v2/supp-5

Clean data of Tibetan sheep in groups HS3

The 4 sequences in the file are represented 4 Tibetan sheep in group HS3.

DOI: 10.7287/peerj.preprints.27807v2/supp-6

Clean data of Tibetan sheep in groups HS4

The 4 sequences in the file are represented 4 rumen samples of Tibetan sheep in group HS4.

DOI: 10.7287/peerj.preprints.27807v2/supp-7

Clean data of Tibetan sheep in groups HS5

The 4 sequences in the file are represented 4 Tibetan sheep in group HS5.

DOI: 10.7287/peerj.preprints.27807v2/supp-8

The nutritional composition of the whole diets among different treatments (% DM basis)

a The tratments HS1, HS2, HS3, HS4 and HS5 refer to the C:F ratios of 0:100, 15:85, 30:70, 45:55 and 60:40, respectively. b DM= dry matter; CP= crude protein; EE= ether extract; ADF= acid detergent fibre; NDF= neutral detergent fibre. c Manufactured by Menyuan Yongxing Ecological Agricultural Development Co., Ltd. Contained corn (48%), wheat (30%), soybean meal (7%), colza cake (6%)cottonseed meal (5%), , salt (1%), pre-mix (1%),CaHPO4 (1%) and CaCO 3 (1%).

DOI: 10.7287/peerj.preprints.27807v2/supp-9

The effects of different dietary C: F ratios on the growth performance of Tibetan sheep

a The treatments HS1, HS2, HS3, HS4 and HS5 refer to concentrate-to-forage ratios of 0:100,15:85, 30:70, 45:55 and 60:40. DMI=dry matter intake; DM consume= DMI×t, t is experimental time(d), BW=body weight; ADG= average daily BW gain . FCR= feed conversion ratio. Values in the same row with different superscripts are significantly different (P<0.05).

DOI: 10.7287/peerj.preprints.27807v2/supp-10

The effects of different dietary C: F ratios on the rumen fermentation parameters of Tibetan sheep

a The Treatments HS1, HS2, HS3, HS4 and HS5 refer to concentrate to forage ratios of 0:100, 15:85, 30:70, 45:55 and 60:40, respectively. b Values in the same row with different superscripts are significantly different (P<0.05).

DOI: 10.7287/peerj.preprints.27807v2/supp-11

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Hongjin Liu conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper.

Tianwei xu conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Shixiao Xu conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Li Ma approved the final draft, dr Li Ma helped to collect the samples and review the manuscript.

Xueping Han approved the final draft, dr Li Ma helped to review the manuscript.

Xungang Wang approved the final draft.

Xiaoling Zhang approved the final draft.

Linyong Hu approved the final draft, dr Linyong Hu helped to collect the samples and review the manuscript.

Na Zhao approved the final draft, dr Na Zhao helped to collect the samples and review the manuscript.

Yongwei Chen approved the final draft, mr Yongwei Chen helped to collect the samples.

Li Pi contributed reagents/materials/analysis tools, approved the final draft.

Xinquan Zhao approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All animal care procedures were consistent with the provision of the Institution of Animal Care and the Ethics Committee of the Northwest Institute of Plateau Biology, Chinese Academy of Sciences (NWIPB20160302).

Data Deposition

The following information was supplied regarding data availability:

The sequencing data for the 16S rRNA genes are publicly available in the NCBI (https://www.ncbi.nlm.nih.gov/) Short Read Archive under accession NO. PRJNA477411

https://www.ncbi.nlm.nih.gov/bioproject/PRJNA477411.

Funding

This work was supported by National Key R&D Plan (NO. 2016YFC0501905, NO. 2016YFC0501805), “Strategic Priority Research Program” of CAS, “Key Technology Support Program” of Qinghai province (No. 2018-S-2, 2015-SF-A4-2, 2016-NK-148, 2017-SF-A6, 2017-NK-153) and Qinghai innovation platform construction project (No. 2017-ZJ-Y20) and NSFC (No. 31402120). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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