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SNARE (Soluble N - ethylmaleimide - sensitive - factor attachment protein receptor) proteins are mainly mediated eukaryotic cell membrane fusion of vesicles transportation, also play an important role in plant resistance to fungal infection. In this study, 1342 SNARE proteins were identified in 18 plants. According to the reported research, it was splited into 5 subfamilies (Qa, Qb, Qc, Qb+Qc and R) and 21 classes. The number of SYP1 small classes in Qa is the largest (227), and Qb+Qc is the smallest (67). Secondly, through the analysis of phylogenetic trees, it was shown that the most SNAREs of 18 plants were distributed in 21 classes. Further analysis of the genetic structure showed that there was a large difference of 21 classes, and the structure of the same group was similar except for individual genes. In wheat, 173 SNARE proteins were identified, except for the first homologous group (14), and the number of others homologous groups were similar. The 2000bp promoter region upstream of wheat SNARE gene was analyzed, and a large number of W-box, MYB and disease-related cis-acting elements were found. The qRT-PCR results of the SNARE gene showed that the expression patterns of the same subfamily were similar in one wheat varieties. The expression patterns of the same gene in resistant/sensitive varieties were largely different at 6h after infection. This results might indicate that early stages of the SNARE protein in pathogen infection play an important role. In this study, the identification and expression analysis of the SNARE protein provides a theoretical basis for future studies on the function of the SNARE protein and wheat resistance to powdery mildew.