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The qPCR and loop mediated isothermal amplification for rapid detection of Ustilago tritici

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23 days ago
The qPCR and loop mediated isothermal amplification for rapid detection of Ustilago tritici https://t.co/4dLY5p4C45 https://t.co/xpK3xMkHLA
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Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Hanwen Yan conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, collection and cultivation of experimental strains.

Jian Zhang conceived and designed the experiments, performed the experiments, fluorescence quantitative PCR operation and data collation.

Dongfang Ma contributed reagents/materials/analysis tools, approved the final draft, modifications and suggestions for the article.

Junliang Yin contributed reagents/materials/analysis tools, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

Data is available at NCBI GEO, accession numbers: AJ236139.1 and DQ132988.1, ITS-4/ITS-5, JN367334.1, AF135430.1, JN367307.1, KP256755.1.

Funding

This work was funded by the “National Key R& D Program of China (2018YFD0200500)”, “Open Project Program of Key Laboratory of Integrated Pest Management Crops in Central China, Ministry of Agriculture. P. R. China/Hubei Key Laboratory of Crop Disease, Insect Pests and Weeds Control (2017ZTSJJ7)”. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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