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Kristiana R, Bedoux G, Pals G, Mudianta IW, Taupin L, Marty C, Asagabaldan MA, Ayuningrum D, Trianto A, Bourgougnon N, Radjasa OK, Sabdono A.2019. Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia. PeerJ Preprints7:e27745v1https://doi.org/10.7287/peerj.preprints.27745v1
The aim of this work was to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-HSV-1 activities of bio-compounds. Fifteen species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated 2 isolates of active bacteria. Ethyl acetate extracts from supernatants, indicating secreted compounds, showed an inhibition zone against MRSA at concentrations of 500-1000µg/ml. DNA sequence analysis showed that the strainKJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, the strain NP31-01 from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of P. rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5-1000 µg/ml. No anti-HSV-1 was observed. This is the first reported study describing research on anti-MRSA, cytotoxicity and anti-HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced and secreted byPseudoalteromonas rubra and Virgibacillus salarius, symbionts of Nudibranch, had potential anti-MRSA activity. Extracts from P. rubra showed cytotoxic effects on Vero cells, whereas extracts from V. salarius did not show cytotoxic effects. Three compounds were identified by LC/MS after purification from culture supernatant.
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Cytotoxic and antiviral activity of Virgibacillus salarius