Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

Rhesi Kristiana; Gilles Bedoux; Gerard Pals; I Wayan Mudianta; Laure Taupin; Christel Marty; Meezan Ardhanu Asagabaldan; Diah Ayuningrum; Agus Trianto; Nathalie Bourgougnon; Ocky Karna Radjasa; Agus Sabdono

doi:10.7287/peerj.preprints.27745v1

Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

Rhesi Kristiana ¹, Gilles Bedoux², Gerard Pals³, I Wayan Mudianta⁴, Laure Taupin², Christel Marty², Meezan Ardhanu Asagabaldan¹, Diah Ayuningrum¹, Agus Trianto⁵, Nathalie Bourgougnon², Ocky Karna Radjasa⁵, Agus Sabdono⁵

1 Department of Coastal Resource Management, Universities Diponegoro, Semarang, Central Java, Indonesia

2 Laboratory of Marine Biotechnology and Chemistry, Université de Bretagne Sud, Vannes, Bretagne, France

3 Center for Connective Tissue research, VU University medical center, Amsterdam, Netherlands

4 Chemical Analysis Study Program, Universitas Pendidikan Ganesha, Singaraja, Bali, Indonesia

5 Department of Marine Sciences, Faculty of Fisheries and Marine Sciences, Universities Diponegoro, Semarang, Central Java, Indonesia

DOI: 10.7287/peerj.preprints.27745v1

Published: 2019-05-20
Accepted: 2019-05-20

Subject Areas: Biodiversity, Biotechnology, Microbiology, Infectious Diseases, Aquatic and Marine Chemistry
Keywords: Nudibranch, Virgibacillus salarius, Pseudoalteromonas rubra, antibiotic, HSV-1, Anti-MRSA activity

Copyright: © 2019 Kristiana et al.
Licence: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.

Cite this article: Kristiana R, Bedoux G, Pals G, Mudianta IW, Taupin L, Marty C, Asagabaldan MA, Ayuningrum D, Trianto A, Bourgougnon N, Radjasa OK, Sabdono A. 2019. Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia. PeerJ Preprints 7:e27745v1 https://doi.org/10.7287/peerj.preprints.27745v1

Abstract

The aim of this work was to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-HSV-1 activities of bio-compounds. Fifteen species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated 2 isolates of active bacteria. Ethyl acetate extracts from supernatants, indicating secreted compounds, showed an inhibition zone against MRSA at concentrations of 500-1000µg/ml. DNA sequence analysis showed that the strainKJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, the strain NP31-01 from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of P. rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5-1000 µg/ml. No anti-HSV-1 was observed. This is the first reported study describing research on anti-MRSA, cytotoxicity and anti-HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced and secreted byPseudoalteromonas rubra and Virgibacillus salarius, symbionts of Nudibranch, had potential anti-MRSA activity. Extracts from P. rubra showed cytotoxic effects on Vero cells, whereas extracts from V. salarius did not show cytotoxic effects. Three compounds were identified by LC/MS after purification from culture supernatant.