Off-line and on-line optical monitoring of microalgal growth

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1 Cátedras CONACYT-El Colegio de la Frontera Sur, Chetumal, Quintana Roo, México
2 Departamento de Física Aplicada, Cinvestav Unidad Mérida, Mérida, Yucatán, México
3 Estación para la Recepción de Información Satelital ERIS-Chetumal, El Colegio de la Frontera Sur, Chetumal, Quintana Roo, México
DOI
10.7287/peerj.preprints.27744v1
Published
Accepted
Subject Areas
Bioengineering, Microbiology, Freshwater Biology, Natural Resource Management, Food, Water and Energy Nexus
Keywords
Chlamydomonas reinhardtii, Chlorophyll fluorescence, Chlorophyll optical density, Fluorescence ratio, Chl a fluorescence images, C. reinhardtii cultures growth
Copyright
© 2019 Lazcano-Hernandez et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Lazcano-Hernandez H, Aguilar G, Dzul G, Patiño R, Arellano-Verdejo J. 2019. Off-line and on-line optical monitoring of microalgal growth. PeerJ Preprints 7:e27744v1

Abstract

The growth of Chlamydomonas reinhardtii microalgae cultures was successfully monitored, from classic off-line optical techniques (optical density and fluorescence) to on-line analysis of digital images. In this study, it is shown that the chlorophyll fluorescence ratio F685/F740 has a linear correlation with the logarithmic concentration of microalgae. Moreover, with digital images, the biomass concentration was correlated with: the luminosity of the images through an exponential equation, and the length of penetration of a superluminescent blue beam (λ=440 nm), through an inversely proportional function. Outcomes of this study are useful to monitor both research and industrial microalgae cultures.

Author Comment

This is a submission to PeerJ for review.

Supplemental Information

Of-line fluorescence measurements of C. reinhardtii cultures

raw data of: Of-line fluorescence measurements of C. reinhardtii cultures, corresponding to experiments A and B

DOI: 10.7287/peerj.preprints.27744v1/supp-1

Of-line optical density measurements of C. reinhardtii cultures

Raw data of: Of-line optical density measurements of C. reinhardtii cultures, corresponding to experiments A and B

DOI: 10.7287/peerj.preprints.27744v1/supp-2

Luminosity and bean penetration measurements in PBR

Raw data of Luminosity and Optical density vs bean penetration measurements in PBR.

DOI: 10.7287/peerj.preprints.27744v1/supp-3