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Pangenomic type III effector database of the plant pathogenic Ralstonia spp.

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RT @nemopeeters:
6 days ago
RT @nemopeeters:
RT @nemopeeters:
6 days ago
Pangenomic type III effector database of the plant pathogenic Ralstonia spp. https://t.co/9MwnYT9Uwl https://t.co/Z8OhnbwLpB
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Supplemental Information

150-strains associated metadata

DOI: 10.7287/peerj.preprints.27726v1/supp-1

mutS alignment and phylogenetic tree on the set of 140 different strains

A neighbor-joining tree was build using the mutS from Ralstonia pickettii as an outgroup. Bootstrap were performed on 100 replicates, only support higher than 50% displayed in the consensus tree.

DOI: 10.7287/peerj.preprints.27726v1/supp-2

Un-cropped western-blot version of Fig. 2

DOI: 10.7287/peerj.preprints.27726v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Cyrus Raja Rubenstein Sabbagh conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Sébastien Carrère conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Fabien Lonjon conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Fabienne Vailleau conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Alberto P Macho conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Stephane Genin conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Nemo Peeters conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

The raw data, i.e. the genomes of the 155 strains are publicly available in GenBank and references thereof have been entered in our Table S1, which also contains all the metadata necessary for redoing the analysis. On our https://iant.toulouse.inra.fr/T3E (maintained now since 2013, and will continue to be), one can download all the data using specific queries. Raw data files are also available (whole genome prediction files).

Funding

CRRS was funded by grants from Lebanon (the municipality of Nabatieh and the association for scientific orientation and specialization). APM is supported by the Shanghai Center for Plant Stress Biology (Chinese Academy of Sciences) and the Chinese 1000 Talents Program. We also received funding from the Laboratoire d’Excellence (LABEX) TULIP (ANR-10-LABX-41). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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