The key genes involved in herpes simplex virus-1 corneal infection-induced acute hepatitis
- Published
- Accepted
- Subject Areas
- Bioinformatics, Genetics, Infectious Diseases, Ophthalmology
- Keywords
- Hepatitis, Diagnosis, Biomarkers, Time series expression analysis, Herpes simplex virus-1
- Copyright
- © 2019 Hu et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2019. The key genes involved in herpes simplex virus-1 corneal infection-induced acute hepatitis. PeerJ Preprints 7:e27724v1 https://doi.org/10.7287/peerj.preprints.27724v1
Abstract
Background: Viral keratitis is mainly induced by herpes simplex virus (HSV). HSV-1 infection-induced acute hepatitis associates with immunodeficiency. Related biomarkers have not been systemically identified till now. This study was designed to explore the molecular mechanisms and potential biomarkers of HSV-1 infection-induced acute hepatitis.
Methods: Microarray dataset GSE35943, including the liver tissues infected by HSV-1 (1, 3, 5, and 7 days post infection) and the corresponding control tissues, was extracted from Gene Expression Omnibus database. The differentially expressed genes (DEGs) were identified using and were clustered using time series expression analysis. DEG-associated KEGG pathways were called using online DAVID tool. Using Cytoscape software, protein-protein interaction (PPI) network was built and significant network modules were identified.
Results: A total of 2909 DEGs grouping into 3 clusters with similar gene expression profiles were obtained. The DEGs were involved in immune-associated functional terms and pathways like natural killer cell mediated cytotoxicity and antigen processing and presentation. DEGs including PIK3R1, PIK3CD, PLCG2, PTPN6, LCK, RAC2, and PLK1 had higher degrees in the PPI network and 8 significant modules
Conclusion: PIK3R1, PIK3CD, PLCG2, PTPN6, LCK, RAC2 and PLK1 were identified to be associated with HSV-1 corneal infection-induced hepatitis, and might be potential clinical biomarkers for the diagnosis of HSV-1-induced hepatitis.
Author Comment
This is a submission to PeerJ for review.