Visitors   Views   Downloads
NOT PEER-REVIEWED
"PeerJ Preprints" is a venue for early communication or feedback before peer review. Data may be preliminary.

Supplemental Information

mtDNA damage and repair in other octocorals species

Quantification of mtDNA lesion frequency (MLF) per 10 kb DNA by SLR-qPCR amplification of total DNA from Sinularia sp. and Briareum sp. exposed to 5 mM H2O2 for 30 min (designated as ‘Treatment’) followed by recovery for 1h (designated as ‘Rec-1H’). Data represents the mean ± s.e.m. of three replicates.

DOI: 10.7287/peerj.preprints.2770v1/supp-1

Melting curve analysis of target and reference genes

A. Melting curves for the reference genes : (A) ACTB, (B) TUBB, (C) RPL12, and (D) SRP54.

B. Melting curves for the target genes: (E) HSP70, (F) GPX, (G) CuZnSOD, (H) COI, and (I) mtMutS.

DOI: 10.7287/peerj.preprints.2770v1/supp-2

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Gaurav G Shimpi conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Sergio Vargas conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Gert Wörheide conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The CuZnSOD and GPX sequences described here are accessible via ENA accession numbers LT717245 and LT717246.

Data Deposition

The following information was supplied regarding data availability:

The raw data has been supplied as a supplementary files.

Funding

The research was funded by DAAD (German Academic Exchange Service) PhD scholarship awarded to Gaurav G. Shimpi. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


Add your feedback

Before adding feedback, consider if it can be asked as a question instead, and if so then use the Question tab. Pointing out typos is fine, but authors are encouraged to accept only substantially helpful feedback.

Some Markdown syntax is allowed: _italic_ **bold** ^superscript^ ~subscript~ %%blockquote%% [link text](link URL)
 
By posting this you agree to PeerJ's commenting policies