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Diets containing edible cricket support a healthy gut microbiome in dogs

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20 days ago
Great work @hollyhganz + @JessicaJarett Just published @PeerJPreprints on how cricket protein in dog food affects the microbiome. Any feedback is welcome! https://t.co/pw04xFRJgE @jiminysforpets @animalbiome #FoodScienceandTechnology #Microbiology #VeterinaryMedicine https://t.co/oG2K5j46S2
20 days ago
What is the culinary relevance? "Diets containing edible cricket support a healthy gut microbiome in dogs" https://t.co/mwm2kwwR3Z https://t.co/X8s4Wq01CH
25 days ago
Diets containing edible cricket support a healthy gut microbiome in dogs https://t.co/pNgwqh9NEF https://t.co/IsLiSzbXno
Diets containing edible cricket support a healthy gut microbiome in dogs https://t.co/jTRJBoWaMc https://t.co/FUkuyvu4S2
@jessicajarett and I just published @PeerJPreprints on how cricket protein in dog food affects the microbiome. Any feedback is welcome! https://t.co/v8f2Q2r1T3 @jiminysforpets @animalbiome #FoodScienceandTechnology #Microbiology #VeterinaryMedicine
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Supplemental Information

Raw and rarefied ASV tables

The raw ASV table contains one sample which was dropped from all analyses due to a highly divergent community relative to other samples. The rarefied ASV table is subsampled to a depth of 26,679 reads per sample.

DOI: 10.7287/peerj.preprints.27677v1/supp-1

Nutritional analysis of diets by dry weight

DOI: 10.7287/peerj.preprints.27677v1/supp-2

Metadata, read counts, and observed ASVs for all samples

DOI: 10.7287/peerj.preprints.27677v1/supp-3

Two-way analysis of variance of read counts

P-values in bold are considered statistically significant.

DOI: 10.7287/peerj.preprints.27677v1/supp-4

Linear mixed-effects model results for alpha and beta diversity metrics

DOI: 10.7287/peerj.preprints.27677v1/supp-5

Analysis of composition of microbiomes at a p-value of 0.05, for ASVs with at least 500 total reads and minimum prevalence of 10% of samples

DOI: 10.7287/peerj.preprints.27677v1/supp-6

Sequencing reads per sample, grouped by date of sampling

DOI: 10.7287/peerj.preprints.27677v1/supp-7

Phylum-level composition of gut microbiomes in dogs consuming diets containing different amounts of cricket meal, sampled longitudinally over the course of 29 days

Low-abundance phyla Euryarchaeota, Synergistetes, Spirochaetes, Deferribacteres are not shown.

DOI: 10.7287/peerj.preprints.27677v1/supp-8

Genus-level composition of gut microbiomes in dogs consuming diets containing different amounts of cricket meal, sampled longitudinally over the course of 29 days

Only the 15 most abundant genera are shown.

DOI: 10.7287/peerj.preprints.27677v1/supp-9

Pielou’s evenness of gut microbiomes of dogs consuming diets containing different amounts of cricket meal, averaged across 8 dogs per diet and sampled longitudinally over the course of 29 days

DOI: 10.7287/peerj.preprints.27677v1/supp-10

Richness (observed ASVs) of gut microbiomes of dogs consuming diets containing different amounts of cricket meal, averaged across 8 dogs per diet and sampled longitudinally over the course of 29 days

DOI: 10.7287/peerj.preprints.27677v1/supp-11

Ratio of Firmicutes to Bacteroidetes in gut microbiomes of dogs consuming diets containing different amounts of cricket meal, averaged across 8 dogs per diet and sampled longitudinally over 29 days

DOI: 10.7287/peerj.preprints.27677v1/supp-12

Additional Information

Competing Interests

Anne Carlson is the founder and CEO of Jiminy’s, a company that makes dog treats containing edible cricket. Holly H. Ganz and Jessica K. Jarett are employees of AnimalBiome, a company that performs gut microbiome testing for cats and dogs.

Author Contributions

Jessica K Jarett analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Anne Carlson conceived and designed the experiments, contributed reagents/materials/analysis tools, approved the final draft.

Mariana C Rossoni Serao analyzed the data, approved the final draft.

Jessica Strickland conceived and designed the experiments, performed the experiments, approved the final draft.

Laurie Serfilippi performed the experiments, approved the final draft.

Holly H Ganz performed the experiments, contributed reagents/materials/analysis tools, approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Summit Ridge Farms's Institutional Animal Care and Use Committee provided full approval for this research.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The 16S rRNA PCR amplicon sequences are available at the NCBI sequence read archive via accession PRJNA525542.

https://www.ncbi.nlm.nih.gov/bioproject/PRJNA525542

Data Deposition

The following information was supplied regarding data availability:

The raw ASV table and rarefied ASV table are provided in Supplemental Data S1. The raw ASV table contains one sample which was dropped from all analyses due to a highly divergent community relative to other samples. The rarefied ASV table is subsampled to a depth of 26,679 reads per sample.

Funding

Jiminy's provided financial support for costs related to the diet trial, AnimalBiome provided financial support for sequence data analysis, and both companies provided financial support for sequencing. Anne Carlson of Jiminy's and Jessica Strickland designed the study protocol. Jiminy's and AnimalBiome decided to prepare and publish a manuscript, and AnimalBiome employees analyzed the data and prepared the manuscript.


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