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Genome-wide identification, phylogenetic and biochemical analysis of the APYRASE family members, and gene expression analysis in response to the abiotic and biotic stresses in bread wheat (Triticum aestivum)

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64 days ago
Genome-wide identification, phylogenetic and biochemical analysis of the APYRASE family members, and gene expression analysis in response to the abiotic and biotic stresses in bread wheat (Triticum aestivum) https://t.co/gXbw8EaIcN https://t.co/Q0sy6XwGCr
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Supplemental Information

Structure analysis of the wheat APYs

The Apyrase Conserved Regions were marked with red box.

DOI: 10.7287/peerj.preprints.27674v1/supp-1

Conserved motif analysis of the wheat APYs

DOI: 10.7287/peerj.preprints.27674v1/supp-2

Membrane spanning Motif analysis of the Arabidopsis APYs

DOI: 10.7287/peerj.preprints.27674v1/supp-3

Raw data of experiments in Figure 6, 7 and 8

DOI: 10.7287/peerj.preprints.27674v1/supp-4

CDS sequence of the 24 wheat APY candidates

DOI: 10.7287/peerj.preprints.27674v1/supp-5

List of the species used in the phylogenetic analysis

DOI: 10.7287/peerj.preprints.27674v1/supp-6

Information of the primers used in this study

DOI: 10.7287/peerj.preprints.27674v1/supp-7

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Wenbo Liu conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Jun Ni conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Faheem Shah performed the experiments, analyzed the data, prepared figures and/or tables.

Kaiqin Ye performed the experiments, contributed reagents/materials/analysis tools.

Hao Hu performed the experiments.

Qiaojian Wang analyzed the data.

Dongdong Wang analyzed the data, contributed reagents/materials/analysis tools.

Yuanyuan Yao contributed reagents/materials/analysis tools.

Shengwei Huang contributed reagents/materials/analysis tools.

Jinyan Hou contributed reagents/materials/analysis tools.

Chenghong Liu contributed reagents/materials/analysis tools.

Lifang Wu approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

The raw measurements are provided in the supplementary table 1. The raw data shows the CDS sequence of the wheat APYRASEs

Funding

This work was supported by the National Key R&D Program of China (2018YFD0300901 & 2018YFD0502001),the Science and Technology Service program of Chinese Academy of Sciences (KFJ-STS-ZDTP-054), the key program of 13th five-year plan, CASHIPS(No. kp-2017-21), and Shanghai Agriculture Applied Technology Development Program (G2015060104). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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