Analysis of virulence factors and emm typing of Streptococcus pyogenes clinical isolates.
- Published
- Accepted
- Subject Areas
- Microbiology, Infectious Diseases
- Keywords
- GAS, emm typing, emm pattern, virulence factors, Real-Time PCR
- Copyright
- © 2019 Sambrano et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2019. Analysis of virulence factors and emm typing of Streptococcus pyogenes clinical isolates. PeerJ Preprints 7:e27649v1 https://doi.org/10.7287/peerj.preprints.27649v1
Abstract
Background: Streptococcus pyogenes, a Group A streptococci (GAS), is an important human pathogen that causes a wide range of infections. Methods: Twenty five clinical isolates of S. pyogenes were submitted to an emm typing and to a Real-time PCR analysis for 23 important virulence factors. Results: Fourteen emm types were found and the emm1 type was the most prevalent. The majority of the isolates were classified as emm pattern E, followed by A-C3. No pattern D was found. Among the virulence factors, the most prevalent were SpeG, Slo, C5a-peptidase and SPNA. Phage encoded virulence genes were also found among the strains, such as mf-2, SpeJ and SpeL. Discussion: The emm1 type was the most prevalent while the 13 others M types were distributed along the strains. No tissue tropism was found on the isolates. The virulence factors analysis demonstrated that chromosomally and phage-encoded genes were found, which confers a potential for high virulent micro-organisms.
Author Comment
This is a preprint submission to PeerJ Preprints.