Experiment 1 Bioanalyzer results
Extracted DNA was run on a Bioanalyzer High Sensitivity DNA Assay for all samples and successful libraries (see methods) are shown. Each sample had 15 PCR cycles. Upper marker designated with purple and lower marker with green.
Relative abundance of vOTUs across 2 bog and 4 fen viromes with variable genome coverage cutoffs (Experiment 1)
Four heatmaps are shown comparing the relative abundances of the 516 vOTUs with different thresholds on the minimum percentage of genome covered (10%, 20%, 30%, and 75%). The relative abundance was normalized per Gbp of metagenome and log10-transformed. All mapping used a minimum nucleotide identify of 90%.
Relative abundance of vOTUs across 12 palsa viromes with variable genome coverage cutoffs (Experiment 2)
Six heatmaps are shown comparing the relative abundances of the 66 vOTUs with different thresholds on the minimum percentage of genome covered, increasing in increments of 10 (0–60%). The relative abundance was normalized per Gbp of metagenome and log10-transformed. All mapping used a minimum nucleotide identify of 90%.
Diversity metrics of vOTUs
(A) Richness (R), Pielou's evenness index (J), and Shannon’s Diversity index (H) were calculated for each virome and the viromes are plotted by habitat. Within each habitat the viromes are denoted by a circle, but displayed differently per treatment. For Experiment 1 (bog and fen), viromes are colored green for PowerSoil and blue for Wizard DNA extractions methods. Experiment 2 (palsa) viromes are outlined in red for heat treated samples or blue for bead-beating samples. The marker is filled in for samples that were CsCl purified. (B) A principal coordinate analysis of the viromes by normalized relative abundance of the 66 vOTUs from Experiment 2 based on their Bray-Curtis dissimilarity. Viromes distinguished by soil core, purification (+CsCl outlined in red), and lysis method.
ssDNA vOTUs from both Experiments
The detected ssDNA virus sequences (see methods) were clustered at 95% average nucleotide identify (ANI) across 85% of their contig length, resulting in 13 vOTUs from the 18 viromes. The ssDNA viruses from each experiment are listed along with their corresponding marker gene and habitat of origin.