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Supplemental Information

Supplementary data 1: Exons sequences corresponding to the 134 markers selected for the empirical study and the complete pools of marker selected using each of the methods compared (fasta format)

DOI: 10.7287/peerj.preprints.2763v3/supp-1

Supplementary data 2: Sequences alignments

DOI: 10.7287/peerj.preprints.2763v3/supp-2

Summary table of markers

Supplementary data 3 - Table documenting markers as represented in Supplementary data 1-4.

DOI: 10.7287/peerj.preprints.2763v3/supp-3

Gene trees from empirical data

Supplementary data 4 - Gene trees inferred under RAxML

DOI: 10.7287/peerj.preprints.2763v3/supp-4

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Malvina Kadlec conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables.

Dirk U Bellstedt contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Nicholas C Le Maitre contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Michael D Pirie conceived and designed the experiments, wrote the paper.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

We thank Cape Nature and South Africa National Parks for assistance with permits (Cape Nature: 0028-AAA008-00134; South Africa National Parks: CRC-2009/007-2014)

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Sequence alignments are available in a supplemental file. Raw sequence reads are available from NCBI: PRJNA388814, https://www.ncbi.nlm.nih.gov/bioproject/388814

Data Deposition

The following information was supplied regarding data availability:

GitHub

9765d11

https://github.com/MaKadlec/Select-Markers/tree/AllMarkers

GitHub

31409eb

https://github.com/MaKadlec/Select-Markers/tree/BestMarkers.py

Funding

The authors acknowledge funding from the Deutsche Forschungsgemeinschaft (DFG; PI1169/1-1 to MDP); South African National Research Foundation (NRF; DUB and MDP). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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