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An application of PCR-RFLP species identification assay for environmental DNA detection

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99 days ago
An application of PCR-RFLP species identification assay for environmental DNA detection https://t.co/9MK8c8QXbp https://t.co/rib2uNR4g1
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Supplemental Information

PCR-RFLP and gel electrophoresis of field experiment (A)

DOI: 10.7287/peerj.preprints.27601v1/supp-1

Supplementary Table 1: Sample infomation

n/a qPCR not conducted; Rj = R. japonica; Ro = R. ornativentris; Rtt = R. t. tagoi

DOI: 10.7287/peerj.preprints.27601v1/supp-2

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Takeshi Igawa conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Teruhiko Takahara conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Quintin Lau performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Shohei Komaki performed the experiments, approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Hiroshima University Animal Research Committee provided full approval for this research (Approval number: G17-4)

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Permission to enter the survey area was obtained from the Iitate Vilage and Namine Town.

Data Deposition

The following information was supplied regarding data availability:

raw gel image are provided in the files 1 - 6.

Funding

This work was supported by a Scientific Research Grant from the Kurita Water, and Environment Foundation Grant and by the Environment Research and Technology Development Fund (4RF1302, 4-1602 and 4-1503) of the Ministry of the Environment, Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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